{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:37:03Z","timestamp":1792654623338,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,12,20]]},"abstract":"<jats:p>A study was made of the effects of di- and trivalent cations on homomeric rho 1-type gamma-aminobutyrate (GABA rho 1) receptors expressed in Xenopus oocytes after injection of mRNA coding for the GABA rho 1 subunit. GABA elicited large currents with a Kd approximately 1 microM. The properties of these GABA rho 1 receptors were similar to those of native bicuculline-resistant GABA receptors expressed by retinal mRNA. GABA rho 1 currents showed very little desensitization, were blocked by picrotoxin but not by bicuculline, and were not modulated by barbiturates, benzodiazepines, or beta-carbolines. Zn2+ reversibly decreased GABA rho 1 responses (IC50 = 22 microM). Other divalent cations were also tested and their rank order of potency was: Zn2+ approximately Ni2+ approximately Cu2+ &gt;&gt; Cd2+, whereas Ba2+, Co2+, Sr2+, Mn2+, Mg2+, and Ca2+ showed little or no effect. In contrast, La3+ reversibly potentiated the GABA currents mediated by homomeric GABA rho 1 receptors, with an EC50 = 135 microM and a maximal potentiation of about 100% (GABA, 1 microM; La3+, 1 mM). Other lanthanides showed similar effects (Lu3+ &gt; Eu3+ &gt; Tb3+ &gt; Gd3+ &gt; Er3% &gt; Nd3+ &gt; La3+ &gt; Ce3+). Thus, GABA rho 1 receptors contain sites for cationic recognition, and in particular, Zn2+ may play a role during synaptic transmission in the retina.<\/jats:p>","DOI":"10.1073\/pnas.91.26.12725","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:02:49Z","timestamp":1149066169000},"page":"12725-12729","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["Cationic modulation of rho 1-type gamma-aminobutyrate receptors expressed in Xenopus oocytes."],"prefix":"10.1073","volume":"91","author":[{"given":"D J","family":"Calvo","sequence":"first","affiliation":[{"name":"Department of Psychobiology, University of California, Irvine 92717-4550."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A E","family":"Vazquez","sequence":"additional","affiliation":[{"name":"Department of Psychobiology, University of California, Irvine 92717-4550."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Miledi","sequence":"additional","affiliation":[{"name":"Department of Psychobiology, University of California, Irvine 92717-4550."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,12,20]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.26.12725","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:11:34Z","timestamp":1649859094000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.26.12725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,12,20]]},"references-count":0,"journal-issue":{"issue":"26","published-print":{"date-parts":[[1994,12,20]]}},"alternative-id":["10.1073\/pnas.91.26.12725"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.26.12725","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,12,20]]},"assertion":[{"value":"1994-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}