{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:36:46Z","timestamp":1781102206785,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,2,15]]},"abstract":"<jats:p>We analyzed the T-cell receptor (TCR) V beta\nrepertoire in human immunodeficiency virus type 1 (HIV-1)-infected individuals\nat different stages of disease. To circumvent the effect of HLA and other loci\non the expressed TCR repertoire, we compared the TCR repertoire in nine pairs of\nmonozygotic twins who were discordant for HIV infection. A semiquantitative\npolymerase chain reaction (PCR) assay and flow cytometry enabled us to show\ndistinct differences in the V beta repertoire in the HIV-positive twin compared\nwith the HIV-negative twin. By combining PCR and cytofluorometry, these\ndifferences were restricted to a specific set of TCR V beta segments, with\nmembers of the V beta 13 family perturbed in six out of seven cases and those of\nthe V beta 21 family perturbed in four out of seven cases studied. Most of the\nother V beta families remained unchanged. Our results provide direct evidence\nfor a skewed TCR repertoire in HIV infection.<\/jats:p>","DOI":"10.1073\/pnas.91.4.1529","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:03:29Z","timestamp":1149066209000},"page":"1529-1533","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":55,"title":["Analysis of the T-cell receptor beta-chainvariable-region (V beta) repertoire in monozygotic twins discordant for humanimmunodeficiency virus: evidence for perturbations of specific V beta segmentsin CD4+ T cells of the virus-positive twins."],"prefix":"10.1073","volume":"91","author":[{"given":"N","family":"Rebai","sequence":"first","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Pantaleo","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J F","family":"Demarest","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Ciurli","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Soudeyns","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Adelsberger","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Vaccarezza","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R E","family":"Walker","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R P","family":"Sekaly","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A S","family":"Fauci","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation, National Institute of\rAllergy and Infectious Diseases, National Institutes of Health, Bethesda, MD\r20892."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,2,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.4.1529","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:48:06Z","timestamp":1649857686000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.4.1529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,15]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1994,2,15]]}},"alternative-id":["10.1073\/pnas.91.4.1529"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.4.1529","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,15]]},"assertion":[{"value":"1994-02-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}