{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:22:22Z","timestamp":1781108542869,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,2,15]]},"abstract":"<jats:p>A technique to detect RNA-binding proteins (RBP)\ninvolving hybridization of RNA probe to proteins transferred to a membrane was\nused to study RBP in different mammalian cells and in cells after genotoxic\nstress. With this approach, up to 13 proteins of different sizes were detected\nin crude nuclear extracts by using a viral RNA probe consisting of the\ntrans-activation-responsive (TAR) element of human immunodeficiency virus type 1\n(HIV-1). The TAR RNA probe contains a stem-loop structure found in nascent HIV-1\ntranscripts. A G+C-rich probe with similar structure also bound to many of these\nRBP. Only a 102-kDa protein strongly bound to other RNA probes lacking this\nstructure, while a probe with an A+U-rich stem-loop structure fail to bind most\nRBP, thus indicating a RNA secondary structure preference. The expression of\nthese RBP varied substantially in nine different human and hamster cell lines,\nwith no detectable RBP in two human myeloid lines. Evidence for induction of\nthese RBP was found in six of seven lines after treatment with DNA-damaging\nagents; UV radiation was the most effective agent. In Chinese hamster ovary\ncells, which showed the strongest response, all five RBP present in untreated\ncells rapidly increased in activity after UV irradiation, and eight additional\nRBP were detected. The induction of these RBP by DNA-damaging agents indicates\none or more possible roles for these proteins in the cellular response to\ngenotoxic stress and in viral activation after such stress.<\/jats:p>","DOI":"10.1073\/pnas.91.4.1554","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:03:29Z","timestamp":1149066209000},"page":"1554-1558","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":17,"title":["Induction of RNA-binding proteins in mammalian cellsby DNA-damaging agents."],"prefix":"10.1073","volume":"91","author":[{"given":"F","family":"Carrier","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Pharmacology, National Cancer\rInstitute, NIH, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Gatignol","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology, National Cancer\rInstitute, NIH, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M C","family":"Hollander","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology, National Cancer\rInstitute, NIH, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K T","family":"Jeang","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology, National Cancer\rInstitute, NIH, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"A J","family":"Fornace","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology, National Cancer\rInstitute, NIH, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,2,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.4.1554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:49:03Z","timestamp":1649857743000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.4.1554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,15]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1994,2,15]]}},"alternative-id":["10.1073\/pnas.91.4.1554"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.4.1554","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,15]]},"assertion":[{"value":"1994-02-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}