{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:27:22Z","timestamp":1781101642464,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:p>Low-stringency single specific primer PCR\n(LSSP-PCR) is an extremely simple PCR-based technique that detects single or\nmultiple mutations in gene-sized DNA fragments. A purified DNA fragment is\nsubjected to PCR using high concentrations of a single specific oligonucleotide\nprimer, large amounts of Taq polymerase, and a very low annealing temperature.\nUnder these conditions the primer hybridizes specifically to its complementary\nregion and nonspecifically to multiple sites within the fragment, in a\nsequence-dependent manner, producing a heterogeneous set of reaction products\nresolvable by electrophoresis. The complex banding pattern obtained is\nsignificantly altered by even a single-base change and thus constitutes a unique\n\"gene signature.\" Therefore LSSP-PCR will have almost unlimited\napplication in all fields of genetics and molecular medicine where rapid and\nsensitive detection of mutations and sequence variations is important. The\nusefulness of LSSP-PCR is illustrated by applications in the study of mutants of\nsmooth muscle myosin light chain, analysis of a family with X-linked nephrogenic\ndiabetes insipidus, and identity testing using human mitochondrial\nDNA.<\/jats:p>","DOI":"10.1073\/pnas.91.5.1946","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:05:56Z","timestamp":1149066356000},"page":"1946-1949","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["Sequence-specific \"gene signatures\" can beobtained by PCR with single specific primers at low stringency."],"prefix":"10.1073","volume":"91","author":[{"given":"S D","family":"Pena","sequence":"first","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Barreto","sequence":"additional","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A R","family":"Vago","sequence":"additional","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"De Marco","sequence":"additional","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F C","family":"Reinach","sequence":"additional","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Dias Neto","sequence":"additional","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J","family":"Simpson","sequence":"additional","affiliation":[{"name":"N\u00facleo de Gen\u00e9tica M\u00e9dica de Minas\rGerais (GENE\/MG), Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.5.1946","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:04:14Z","timestamp":1649858654000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.5.1946"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,3]]}},"alternative-id":["10.1073\/pnas.91.5.1946"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.5.1946","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3]]},"assertion":[{"value":"1994-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}