{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:13:50Z","timestamp":1781104430189,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,3,29]]},"abstract":"<jats:p>This laboratory has previously reported that a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (\"12-O-tetradecanoylphorbol 13-acetate\"), induces BJAB cells, a Burkitt lymphoma cell line, to express cellular proteins that bind to the origin of plasmid DNA replication (oriP) of Epstein-Barr virus. These oriP-binding proteins interfere with the EBV-encoded nuclear antigen EBNA-1, which binds to oriP in Raji cells. To further characterize these proteins, a lambda phage expression cDNA library made from phorbol ester-induced BJAB cells was screened for fusion proteins which bind to oriP. Two recombinant phages containing sequences encoding beta-galactosidase fusion proteins and designated lambda-OBP-1 and lambda-OBP-2 were identified. lambda-OBP-1 and lambda-OBP-2 contained 0.43 kbp and 0.61 kbp of BJAB cell cDNA, respectively, of which 395 bp were shared. Using lambda-OBP-1 as probe, two cDNAs of 1.4 kbp and 1.2 kbp, designated OBP-1 and OBP-2, respectively, were isolated. These cDNAs also shared the 395-bp sequence at the 3' end. With these cDNAs as probes, Northern blot analyses of mRNA from BJAB cells gave 1.4, 2.4, and 3.4-kb bands, but a Southern blot of human genomic DNA revealed one band. It is likely that the oriP-binding proteins were derived from spliced mRNA(s) of a gene family.<\/jats:p>","DOI":"10.1073\/pnas.91.7.2843","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:06:33Z","timestamp":1149066393000},"page":"2843-2847","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":22,"title":["The cellular proteins that bind specifically to the Epstein-Barr virus origin of plasmid DNA replication belong to a gene family."],"prefix":"10.1073","volume":"91","author":[{"given":"S","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Tampa Bay Research Institute, St. Petersburg, FL 33716."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Nonoyama","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Tampa Bay Research Institute, St. Petersburg, FL 33716."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,3,29]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.7.2843","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:51:14Z","timestamp":1649857874000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.7.2843"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3,29]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1994,3,29]]}},"alternative-id":["10.1073\/pnas.91.7.2843"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.7.2843","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3,29]]},"assertion":[{"value":"1994-03-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}