{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:07:42Z","timestamp":1792570062703,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,1,3]]},"abstract":"<jats:p>Carotid body-mediated ventilatory increases in response to acute hypoxia are attenuated in animals reared in an hypoxic environment. Normally, O2-sensitive K+ channels in neurosecretory type I carotid body cells are intimately involved in excitation of the intact organ by hypoxia. We have therefore studied K+ channels and their sensitivity to acute hypoxia (PO2 12-20 mmHg) in type I cells isolated from neonatal rats born and reared in normoxic and hypoxic environments. When compared with cells from normoxic rats, K+ current density in cells from hypoxic rats was significantly reduced, whereas Ca2+ current density was unaffected. Charybdotoxin (20 nM) inhibited K+ currents in cells from normoxic rats by approximately 25% but was without significant effect in cells from hypoxic rats. However, hypoxia caused similar, reversible inhibitions of K+ currents in cells from the two groups. Resting membrane potentials (measured at 37 degrees C using the perforated-patch technique) were similar in normoxic and hypoxic rats. However, although acute hypoxia depolarized type I cells of normoxic rats, it was without effect on membrane potential in type I cells from hypoxic animals. Charybdotoxin (20 nM) also depolarized cells from normoxic rats. Our results suggest that type I cells from chronically hypoxic rats, like normoxic rats, possess O2-sensing mechanisms. However, they lack charybdotoxin-sensitive K+ channels that contribute to resting membrane potential in normoxically reared rats, and this appears to prevent them from depolarizing (and hence triggering Ca2+ influx and neurosecretion) during acute hypoxia.<\/jats:p>","DOI":"10.1073\/pnas.92.1.295","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:09:12Z","timestamp":1149066552000},"page":"295-299","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":119,"title":["O2-sensitive K+ currents in carotid body chemoreceptor cells from normoxic and chronically hypoxic rats and their roles in hypoxic chemotransduction."],"prefix":"10.1073","volume":"92","author":[{"given":"C N","family":"Wyatt","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Leeds University, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Leeds University, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Bee","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Leeds University, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Peers","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Leeds University, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1995,1,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.1.295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:08:40Z","timestamp":1649858920000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.1.295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1,3]]}},"alternative-id":["10.1073\/pnas.92.1.295"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.1.295","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1,3]]},"assertion":[{"value":"1995-01-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}