{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:47:37Z","timestamp":1792000057467,"version":"4.4.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,1,17]]},"abstract":"<jats:p>The cDNAs for two membrane guanylyl cyclases, designated E (GC-E) and F (GC-F, were isolated from a rat eye cDNA library. Their deduced topographic structures correspond to known members of the guanylyl cyclase receptor family, containing an extracellular domain, a single membrane-spanning domain, a protein kinase-like domain, and a cyclase catalytic domain. GC-E was expressed in the eye and the pineal gland, whereas GC-F expression was confined to the eye. Overproduction of GC-E and GC-F in COS cells resulted in expression of guanylyl cyclase activity, but ligands known to activate other guanylyl cyclase receptors failed to stimulate enzyme activity. Thus, both GC-E and GC-F remain orphan receptors. Amino acid sequence similarity between GC-E and GC-F in the extracellular region and homology with a cyclase expressed in olfactory neurons and retGC, a rod outer-segment-specific cyclase, suggest that there is another subfamily of guanylyl cyclase receptors, possibly restricted to sensory tissues.<\/jats:p>","DOI":"10.1073\/pnas.92.2.602","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:20:46Z","timestamp":1149067246000},"page":"602-606","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":189,"title":["Two membrane forms of guanylyl cyclase found in the eye."],"prefix":"10.1073","volume":"92","author":[{"given":"R B","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9050."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D C","family":"Foster","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9050."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D L","family":"Garbers","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9050."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H J","family":"F\u00fclle","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9050."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1995,1,17]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.2.602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:09:26Z","timestamp":1649858966000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.2.602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1,17]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,1,17]]}},"alternative-id":["10.1073\/pnas.92.2.602"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.2.602","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1,17]]},"assertion":[{"value":"1995-01-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}