{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:33:41Z","timestamp":1790714021844,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,11,7]]},"abstract":"<jats:p>Microsomal cytochrome P450c17 catalyzes both steroid 17 alpha-hydroxylase activity and scission of the C17-C20 steroid bond (17,20-lyase) on the same active site. Adrenal 17 alpha-hydroxylase activity is needed to produce cortisol throughout life, but 17,20-lyase activity appears to be controlled independently in a complex, age-dependent pattern. We show that human P450c17 is phosphorylated on serine and threonine residues by a cAMP-dependent protein kinase. Phosphorylation of P450c17 increases 17,20-lyase activity, while dephosphorylation virtually eliminates this activity. Hormonally regulated serine phosphorylation of human P450c17 suggests a possible mechanism for human adrenarche and may be a unifying etiologic link between the hyperandrogenism and insulin resistance that characterize the polycystic ovary syndrome.<\/jats:p>","DOI":"10.1073\/pnas.92.23.10619","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:23:10Z","timestamp":1149067390000},"page":"10619-10623","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":334,"title":["Serine phosphorylation of human P450c17 increases 17,20-lyase activity: implications for adrenarche and the polycystic ovary syndrome."],"prefix":"10.1073","volume":"92","author":[{"given":"L H","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of California, San Francisco 94143-0978, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of California, San Francisco 94143-0978, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ohno","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of California, San Francisco 94143-0978, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W L","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of California, San Francisco 94143-0978, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1995,11,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.23.10619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:05:47Z","timestamp":1649858747000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.23.10619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,7]]},"references-count":0,"journal-issue":{"issue":"23","published-print":{"date-parts":[[1995,11,7]]}},"alternative-id":["10.1073\/pnas.92.23.10619"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.23.10619","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,7]]},"assertion":[{"value":"1995-11-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}