{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:14:38Z","timestamp":1792667678649,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"25","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,12,5]]},"abstract":"<jats:p>In establishing the memory B-cell population and maintaining self-tolerance during an immune response, apoptosis mediates the removal of early, low-affinity antibody-forming cells, unselected germinal center (GC) cells, and, potentially, self-reactive B cells. To address the role of the apoptosis-signaling cell surface molecule FAS in the B-cell response to antigen, we have examined the T-cell-dependent B-cell response to the carrier-conjugated hapten (4-hydroxy-3-nitrophenyl)acetyl (NP) in lpr mice in which the fas gene is mutated. High levels of FAS were expressed on normal GC B cells but the absence of FAS did not perturb the progressive decline in numbers of either GC B cells or extrafollicular antibody-forming cells. Furthermore, the rate of formation and eventual size of the NP-specific memory B-cell population in lpr mice were normal. The accumulation of cells with affinity-enhancing mutations and the appearance of high-affinity anti-NP IgG1 antibody in the serum were also normal in lpr mice. Thus, although high levels of FAS are expressed on GC B cells, FAS is not required for GC selection or for regulation of the major antigen-specific B-cell compartments. The results suggest that the size and composition of B-cell compartments in the humoral immune response are regulated by mechanisms that do not require FAS.<\/jats:p>","DOI":"10.1073\/pnas.92.25.11628","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:24:38Z","timestamp":1149067478000},"page":"11628-11632","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":111,"title":["FAS is highly expressed in the germinal center but is not required for regulation of the B-cell response to antigen."],"prefix":"10.1073","volume":"92","author":[{"given":"K G","family":"Smith","sequence":"first","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G J","family":"Nossal","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D M","family":"Tarlinton","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1995,12,5]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.25.11628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:58:35Z","timestamp":1649858315000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.25.11628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12,5]]},"references-count":0,"journal-issue":{"issue":"25","published-print":{"date-parts":[[1995,12,5]]}},"alternative-id":["10.1073\/pnas.92.25.11628"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.25.11628","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12,5]]},"assertion":[{"value":"1995-12-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}