{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:10:00Z","timestamp":1790896200640,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,2,28]]},"abstract":"<jats:p>We describe a simple reproducible system for enrichment and long-term culture of human B-cell progenitors. Enriched CD34+ cord blood mononuclear cells are seeded onto a murine stromal cell line to establish a biphasic culture system. These cultures are characterized by transient growth of myeloid cells followed by outgrowth of cells highly enriched for early B-cell progenitors. Cultures consisting of &gt; 90% early B-lineage cells [expressing CD10, CD19, CD38, and CD45 but lacking CD20, CD22, CD23, and surface IgM] are maintained for &gt; 12 weeks without growth factor addition. Cells remain predominantly germ line at the immunoglobulin locus and express only low levels of cytoplasmic mu chain, terminal deoxynucleotidyltransferase, and recombination-activating gene 1 product. They are unresponsive to the pre-B-cell growth factors interleukin 7 or stem cell factor, or both, suggesting that growth support is provided by a cross-reactive murine stromal cell factor. Cultured B-cell progenitors are generated in large numbers ( &gt; 10(8) cells from a typical cord blood specimen) suitable for use in biochemical analysis and gene-transfer studies. This system should be useful for study of normal and abnormal early human B-lymphopoiesis.<\/jats:p>","DOI":"10.1073\/pnas.92.5.1570","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:27:59Z","timestamp":1149067679000},"page":"1570-1574","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":68,"title":["Long-term culture system for selective growth of human B-cell progenitors."],"prefix":"10.1073","volume":"92","author":[{"given":"D J","family":"Rawlings","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1662."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S G","family":"Quan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1662."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1662."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O N","family":"Witte","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1662."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1995,2,28]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.5.1570","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:53:48Z","timestamp":1649858028000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.5.1570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,2,28]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,2,28]]}},"alternative-id":["10.1073\/pnas.92.5.1570"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.5.1570","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,2,28]]},"assertion":[{"value":"1995-02-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}