{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:58:36Z","timestamp":1781103516339,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,3,14]]},"abstract":"<jats:p>An oligopeptide elicitor from Phytophthora megasperma f.sp. glycinea (Pep-13) that induces phytoalexin accumulation in cultured parsley cells was radioiodinated and chemically cross-linked to its binding site in microsomal and plasma membrane preparations with each of three homobifunctional reagents. Analysis by SDS\/PAGE and autoradiography of solubilized membrane proteins demonstrated labeling of a 91-kDa protein, regardless of which reagent was used. Cross-linking of this protein was prevented by addition of excess unlabeled Pep-13. The competitor concentration found to half-maximally reduce the intensity of the cross-linked band was 6 nM, which is in good agreement with the IC50 value of 4.7 nM, obtained from ligand binding assays. No crosslinking of 125I-labeled Pep-13 was observed by using microsomal membranes from three other plant species, indicating species-specific occurrence of the binding site. Coupling of 125I-Pep-13 to the parsley 91-kDa protein required the same structural elements within the ligand as was recently reported for binding of 125I-Pep-13 to parsley microsomes, elicitor-induced stimulation of ion fluxes across the plasma membrane, the oxidative burst, the expression of defense-related genes, and phytoalexin production. These findings suggest that the 91-kDa protein identified in parsley membranes is the oligopeptide elicitor receptor mediating activation of a multicomponent defense response.<\/jats:p>","DOI":"10.1073\/pnas.92.6.2338","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T09:29:22Z","timestamp":1149067762000},"page":"2338-2342","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":52,"title":["Covalent cross-linking of the Phytophthora megasperma oligopeptide elicitor to its receptor in parsley membranes."],"prefix":"10.1073","volume":"92","author":[{"given":"T","family":"N\u00fcrnberger","sequence":"first","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Abteilung Biochemie, Cologne, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Nennstiel","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Abteilung Biochemie, Cologne, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Hahlbrock","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Abteilung Biochemie, Cologne, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Scheel","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Z\u00fcchtungsforschung, Abteilung Biochemie, Cologne, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1995,3,14]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.6.2338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:06:50Z","timestamp":1649858810000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.6.2338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,14]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,3,14]]}},"alternative-id":["10.1073\/pnas.92.6.2338"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.6.2338","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,14]]},"assertion":[{"value":"1995-03-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}