{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:56:07Z","timestamp":1788375367474,"version":"build-2803163510"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,5,14]]},"abstract":"<jats:p>To analyze the role of alpha4-integrins in lymphoma metastasis, sublines of the T-cell lymphoma LB were generated by retrovirus-mediated gene transfer that differ exclusively in the expression of alpha4-integrins. Using LB-alpha4 and control LB-NTK cells, we demonstrate that expression of alpha4-integrins strongly suppresses metastasis formation of LB lymphoma cells in secondary lymphoid organs such as spleen, mesenteric and peripheral lymph nodes, or Peyer's patches after i.v. injection into syngeneic BALB\/c mice. Moreover, alpha4-integrin expression inhibited development of metastatic tumors in liver, lung, and kidney. Expansion of LB lymphoma cells in bone marrow was not affected by alpha4-integrin expression. In vivo migration assays using 51Cr-labeled lymphoma cells demonstrated that low-metastatic LB-alpha4 cells accumulated with the same efficiency as high-metastatic LB-NTK cells in all target organs examined and were even enriched in mucosal lymphoid organs. Collectively, these results indicate that alpha4-integrins inhibit metastasis formation of lymphoma cells at a stage subsequent to the invasion of target organs.<\/jats:p>","DOI":"10.1073\/pnas.93.10.4821","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:44Z","timestamp":1027679504000},"page":"4821-4826","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":37,"title":["Predominant role of alpha 4-integrins for distinct steps of lymphoma metastasis."],"prefix":"10.1073","volume":"93","author":[{"given":"U","family":"Gosslar","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Jonas","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Luz","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Lifka","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Naor","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Hamann","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Holzmann","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Technical University, Munich, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,5,14]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.93.10.4821","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:08:05Z","timestamp":1649858885000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.93.10.4821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5,14]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1996,5,14]]}},"alternative-id":["10.1073\/pnas.93.10.4821"],"URL":"https:\/\/doi.org\/10.1073\/pnas.93.10.4821","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5,14]]},"assertion":[{"value":"1996-05-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}