{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:35:43Z","timestamp":1791174943086,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,6,25]]},"abstract":"<jats:p>Transcription factor IIH (TFIIH) is a multisubunit protein complex essential for both the initiation of RNA polymerase class II (pol II)-catalyzed transcription and nucleotide excision repair of DNA. Recent studies have shown that TFIIH copurifies with the cyclin-dependent kinase (cdk)-activating kinase complex (CAK) that includes cdk7, cyclin H, and p36\/MAT1. Here we report the isolation of two TFIIH-related complexes: TFIIH* and ERCC2\/CAK. TFIIH* consists of a subset of the TFIIH complex proteins including ERCC3 (XPB), p62, p44, p41, and p34 but is devoid of detectable levels of ERCC2 (XPD) and CAK. ERCC2\/CAK was isolated as a complex that exhibits CAK activity that cosediments with the three CAK components (cdk7, cyclin H, and p36\/MAT1) as well as the ERCC2 (XPD) protein. TFIIH* can support pol II-catalyzed transcription in vitro with lower efficiency compared with TFIIH. This TFIIH*-dependent transcription reaction was stimulated by ERCC2\/CAK. The ERCC2\/CAK and TFIIH* complexes are each active in DNA repair as shown by their ability to complement extracts prepared from ERCC2 (XPD)- and ERCC3 (XPB)-deficient cells, respectively, in supporting the excision of DNA containing a cholesterol lesion. These data suggest that TFIIH* and ERCC2\/CAK interact to form the TFIIH holoenzyme capable of efficiently assembling the pol II transcription initiation complex and directly participating in excision repair reactions.<\/jats:p>","DOI":"10.1073\/pnas.93.13.6482","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:43:20Z","timestamp":1027680200000},"page":"6482-6487","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":100,"title":["Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2\/CAK and TFIIH."],"prefix":"10.1073","volume":"93","author":[{"given":"J T","family":"Reardon","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Gibbs","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Sancar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Hurwitz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z Q","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,6,25]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.93.13.6482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:23:05Z","timestamp":1649863385000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.93.13.6482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,6,25]]},"references-count":0,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1996,6,25]]}},"alternative-id":["10.1073\/pnas.93.13.6482"],"URL":"https:\/\/doi.org\/10.1073\/pnas.93.13.6482","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,6,25]]},"assertion":[{"value":"1996-06-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}