{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:10:00Z","timestamp":1791202200258,"version":"4.2.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,8,20]]},"abstract":"<jats:p>Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis, is a human herpesvirus associated with epithelial cell malignancies (nasopharyngeal carcinoma) as well as B-cell malignancies. Understanding how viral latency is disrupted is a central issue in herpesvirus biology. Epithelial cells are the major site of lytic EBV replication within the human host, and viral reactivation occurs in EBV-associated nasopharyngeal carcinomas. It is known that expression of a single viral immediate-early protein, BZLF1, is sufficient to initiate the switch from latent to lytic infection in B cells. Cellular regulation of BZLF1 transcription is therefore thought to play a key role in regulating the stringency of viral latency. Here we show that, unexpectedly, expression of another viral immediate-early protein, BRLF1, can disrupt viral latency in an epithelial cell-specific fashion. Therefore, the mechanisms leading to disruption of EBV latency appear to be cell-type specific.<\/jats:p>","DOI":"10.1073\/pnas.93.17.9194","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:34:16Z","timestamp":1027679656000},"page":"9194-9199","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":212,"title":["Epstein-Barr viral latency is disrupted by the immediate-early BRLF1 protein through a cell-specific mechanism."],"prefix":"10.1073","volume":"93","author":[{"given":"S","family":"Zalani","sequence":"first","affiliation":[{"name":"Department of Medicine, University of North Carolina 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Holley-Guthrie","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of North Carolina 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kenney","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of North Carolina 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,8,20]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.93.17.9194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:15:26Z","timestamp":1649862926000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.93.17.9194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,20]]},"references-count":0,"journal-issue":{"issue":"17","published-print":{"date-parts":[[1996,8,20]]}},"alternative-id":["10.1073\/pnas.93.17.9194"],"URL":"https:\/\/doi.org\/10.1073\/pnas.93.17.9194","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,8,20]]},"assertion":[{"value":"1996-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}