{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:47:24Z","timestamp":1781106444673,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,9,17]]},"abstract":"<jats:p>Cytotoxic T cells recognize mosaic structures consisting of target peptides embedded within self-major histocompatibility complex (MHC) class I molecules. This structure has been described in great detail for several peptide-MHC complexes. In contrast, how T-cell receptors recognize peptide-MHC complexes have been less well characterized. We have used a complete set of singly substituted analogs of a mouse MHC class I, Kk-restricted peptide, influenza hemagglutinin (Ha)255-262, to address the binding specificity of this MHC molecule. Using the same peptide-MHC complexes we determined the fine specificity of two Ha255-262-specific, Kk-restricted T cells, and of a unique antibody, pSAN, specific for the same peptide-MHC complex. Independently, a model of the Ha255-262-Kk complex was generated through homology modeling and molecular mechanics refinement. The functional data and the model corroborated each other showing that peptide residues 1, 3, 4, 6, and 7 were exposed on the MHC surface and recognized by the T cells. Thus, the majority, and perhaps all, of the side chains of the non-primary anchor residues may be available for T-cell recognition, and contribute to the stringent specificity of T cells. A striking similarity between the specificity of the T cells and that of the pSAN antibody was found and most of the peptide residues, which could be recognized by the T cells, could also be recognized by the antibody.<\/jats:p>","DOI":"10.1073\/pnas.93.19.10338","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"10338-10342","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":32,"title":["Shared fine specificity between T-cell receptors and an antibody recognizing a peptide\/major histocompatibility class I complex."],"prefix":"10.1073","volume":"93","author":[{"given":"A","family":"Stryhn","sequence":"first","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P S","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L O","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Svejgaard","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Holm","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C J","family":"Thorpe","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Fugger","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Buus","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Engberg","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, University of Copenhagen, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,9,17]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.93.19.10338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:59:10Z","timestamp":1649861950000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.93.19.10338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9,17]]},"references-count":0,"journal-issue":{"issue":"19","published-print":{"date-parts":[[1996,9,17]]}},"alternative-id":["10.1073\/pnas.93.19.10338"],"URL":"https:\/\/doi.org\/10.1073\/pnas.93.19.10338","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9,17]]},"assertion":[{"value":"1996-09-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}