{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:50:17Z","timestamp":1781110217648,"version":"3.54.1"},"reference-count":36,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,11,12]]},"abstract":"<jats:p>\n                    The isolation and study of\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    genes\n that are differentially expressed in development, notably in tissues\n associated with the maturation and transmission of the malaria\n parasite, is important for the elucidation of basic molecular\n mechanisms underlying vector\u2013parasite interactions. We have used the\n differential display technique to screen for mRNAs specifically\n expressed in adult males, females, and midgut tissues of blood-fed and\n unfed females. We also screened for mRNAs specifically induced upon\n bacterial infection of larval stage mosquitoes. We have characterized\n 19 distinct cDNAs, most of which show developmentally regulated\n expression specificity during the mosquito life cycle. The most\n interesting are six new sequences that are midgut-specific in the\n adult, three of which are also modulated by blood-feeding. The\n gut-specific sequences encode a maltase, a V-ATPase subunit, a GTP\n binding protein, two different lectins, and a nontrypsin serine\n protease. The latter sequence is also induced in larvae subjected to\n bacterial challenge. With the exception of a mitochondrial DNA\n fragment, the other 18 sequences constitute expressed genomic sequence\n tags, 4 of which have been mapped cytogenetically.\n                  <\/jats:p>","DOI":"10.1073\/pnas.93.23.13066","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"13066-13071","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":81,"title":["Identification and characterization of differentially expressed cDNAs of the vector mosquito,\u2009\n                    <i>Anopheles\u2009gambiae<\/i>"],"prefix":"10.1073","volume":"93","author":[{"given":"George","family":"Dimopoulos","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, Foundation of\r Research and Technology\u2013Hellas, GR-711 10 Heraklion, Crete, Greece;\r Department of Biology, University of Crete, GR-711 10\r Heraklion, Crete, Greece; Istituto di Parassitologia,\r Fondazione Cenci Bolognetti, Universit\u00e0 degli Studi \u201cLa\r Sapienza,\u201d I-00185 Rome, Italy; and European Molecular\r Biology Laboratory, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Richman","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, Foundation of\r Research and Technology\u2013Hellas, GR-711 10 Heraklion, Crete, Greece;\r Department of Biology, University of Crete, GR-711 10\r Heraklion, Crete, Greece; Istituto di Parassitologia,\r Fondazione Cenci Bolognetti, Universit\u00e0 degli Studi \u201cLa\r Sapienza,\u201d I-00185 Rome, Italy; and European Molecular\r Biology Laboratory, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"della\u2009Torre","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, Foundation of\r Research and Technology\u2013Hellas, GR-711 10 Heraklion, Crete, Greece;\r Department of Biology, University of Crete, GR-711 10\r Heraklion, Crete, Greece; Istituto di Parassitologia,\r Fondazione Cenci Bolognetti, Universit\u00e0 degli Studi \u201cLa\r Sapienza,\u201d I-00185 Rome, Italy; and European Molecular\r Biology Laboratory, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fotis\u2009C.","family":"Kafatos","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, Foundation of\r Research and Technology\u2013Hellas, GR-711 10 Heraklion, Crete, Greece;\r Department of Biology, University of Crete, GR-711 10\r Heraklion, Crete, Greece; Istituto di Parassitologia,\r Fondazione Cenci Bolognetti, Universit\u00e0 degli Studi \u201cLa\r Sapienza,\u201d I-00185 Rome, Italy; and European Molecular\r Biology Laboratory, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Louis","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, Foundation of\r Research and Technology\u2013Hellas, GR-711 10 Heraklion, Crete, Greece;\r Department of Biology, University of Crete, GR-711 10\r Heraklion, Crete, Greece; Istituto di Parassitologia,\r Fondazione Cenci Bolognetti, Universit\u00e0 degli Studi \u201cLa\r Sapienza,\u201d I-00185 Rome, Italy; and European Molecular\r Biology Laboratory, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,11,12]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(91)90127-A"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3532325"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1128"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(94)90221-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)18053-F"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.19.4965"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1354393"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.1996.tb00055.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.1991"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/143.3.1307"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.11187"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.17.4147"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.1994.tb00145.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.1993.tb00131.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1118"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.14.5562"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38325-5"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39014-2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(05)80112-5"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53409-8"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31762-9"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.5823"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.199.5.1019"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.15.1787"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.24.9853"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)90926-X"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/303300a0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90384-3"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41926-0"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/978-1-4612-2910-0_4","volume-title":"Advances 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