{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:57:10Z","timestamp":1792673830719,"version":"4.5.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,12,24]]},"abstract":"<jats:p>\n                    A hair cell\u2019s tip links are thought to gate mechanoelectrical\n transduction channels. The susceptibility of tip links to acoustic\n trauma raises questions as to whether these fragile structures can be\n regenerated. We broke tip links with the calcium chelator\n 1,2-bis(\n                    <jats:italic>O<\/jats:italic>\n                    -aminophenoxy)ethane-\n                    <jats:italic>N<\/jats:italic>\n                    ,\n                    <jats:italic>N<\/jats:italic>\n                    ,\n                    <jats:italic>N<\/jats:italic>\n                    \u2032,\n                    <jats:italic>N<\/jats:italic>\n                    \u2032-tetraacetic\n acid and found that they can regenerate, albeit imperfectly, over\n several hours. The time course of tip-link regeneration suggests that\n this process may underlie recovery from temporary threshold shifts\n induced by noise exposure. Cycloheximide does not block tip-link\n regeneration, indicating that new protein synthesis is not required.\n The calcium ionophore ionomycin prevents regeneration, suggesting\n regeneration normally may be stimulated by the reduction in\n stereociliary Ca\n                    <jats:sup>2+<\/jats:sup>\n                    when gating springs rupture and\n transduction channels close. Supporting the equivalence of tip links\n with gating springs, mechanoelectrical transduction returns over the\n same time period as tip links; strikingly, adaptation is substantially\n reduced, even 24 hr after breaking tip links.\n                  <\/jats:p>","DOI":"10.1073\/pnas.93.26.15469","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:44Z","timestamp":1027679504000},"page":"15469-15474","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":201,"title":["Regeneration of broken tip links and restoration of mechanical transduction in\u2009hair\u2009cells"],"prefix":"10.1073","volume":"93","author":[{"given":"Yi-dong","family":"Zhao","sequence":"first","affiliation":[{"name":"Departments of Physiology and Neuroscience, The Johns\r Hopkins University, Baltimore, MD 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebenezer\u2009N.","family":"Yamoah","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Neuroscience, The Johns\r Hopkins University, Baltimore, MD 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter\u2009G.","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Neuroscience, The Johns\r Hopkins University, Baltimore, MD 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,12,24]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/341397a0"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90066-H"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.03-05-00962.1983"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(84)90041-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90343-X"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(87)90089-X"},{"key":"e_1_3_2_7_2","first-page":"63","volume":"2","author":"McNiven A I","year":"1996","unstructured":"A I McNiven, W A Yuhas, P A Fuchs Aud Neurosci 2, 63\u201377 (1996).","journal-title":"Aud Neurosci"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80184-1"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(88)90079-2"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00215434"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(91)90116-Q"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90010-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00179301"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000276384"},{"key":"e_1_3_2_15_2","first-page":"357","volume-title":"Sensory Transduction","author":"Hudspeth A J","year":"1992","unstructured":"A J Hudspeth Sensory Transduction, eds D P Corey, S Roper (Rockefeller Univ. 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