{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:48:09Z","timestamp":1791301689785,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,4,2]]},"abstract":"<jats:p>We reported previously that the human T-cell lymphotrophic virus type I (HTLV-I)-associated adult T-cell leukemia line HuT-102 produces a cytokine designated interleukin (IL) T that requires interleukin (IL) 2 receptor beta-subunit expression for its action. Using anti-cytokine antibodies, we demonstrated that IL-T is identical to the simultaneously described IL-15. When compared to activated monocytes, IL-15 mRNA expression was 6- to 10-fold greater in HuT-102 cells. The predominant IL-15 message from HuT-102 is a chimeric mRNA joining a segment of the R region of the long terminal repeat of HTLV-I and the 5'-untranslated region (UTR) of IL-15. Normally, by alternative splicing, this 118-nucleotide R element represents the most 5' region of several HTLV-I transcripts including tax, rex, and env. The introduction of the R element eliminated over 200 nucleotides of the IL-15 5'-UTR, including 8 of 10 upstream AUGs that are present in normal IL-15 messages. On analysis of the 5'-UTR of normal IL-15, we demonstrated that the presence of these 10 upstream AUGs interferes with IL-15 mRNA translation. Thus, IL-15 synthesis by the adult T-cell leukemia line HuT- 102 involves an increase in IL-15 mRNA transcription and translation secondary to the production of an HTLV-I R element fusion message that lacks many upstream AUGs.<\/jats:p>","DOI":"10.1073\/pnas.93.7.2897","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:43:20Z","timestamp":1027680200000},"page":"2897-2902","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":160,"title":["Interleukin (IL) 15\/IL-T production by the adult T-cell leukemia cell line HuT-102 is associated with a human T-cell lymphotrophic virus type I region \/IL-15 fusion message that lacks many upstream AUGs that normally attenuates IL-15 mRNA translation."],"prefix":"10.1073","volume":"93","author":[{"given":"R N","family":"Bamford","sequence":"first","affiliation":[{"name":"Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1374, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A P","family":"Battiata","sequence":"additional","affiliation":[{"name":"Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1374, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"Burton","sequence":"additional","affiliation":[{"name":"Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1374, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Sharma","sequence":"additional","affiliation":[{"name":"Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1374, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T A","family":"Waldmann","sequence":"additional","affiliation":[{"name":"Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1374, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,4,2]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.93.7.2897","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:16:00Z","timestamp":1649859360000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.93.7.2897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,4,2]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1996,4,2]]}},"alternative-id":["10.1073\/pnas.93.7.2897"],"URL":"https:\/\/doi.org\/10.1073\/pnas.93.7.2897","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,4,2]]},"assertion":[{"value":"1996-04-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}