{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:01:04Z","timestamp":1791039664533,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1996,4,30]]},"abstract":"<jats:p>Semaphorins and collapsins make up a family of conserved genes that encode nerve growth cone guidance signals. We have identified two additional members of the human semaphorin family [human semaphorin A(V) and human semaphorin IV] in chromosome region 3p21.3, where several small cell lung cancer (SCLC) cell lines exhibit homozygous deletions indicative of a tumor suppressor gene. Human semaphorin A(V) has 86% amino acid homology with murine semaphorin A, whereas semaphorin IV is most closely related to murine semaphorin E, with 50% homology. These semaphorin genes are approximately 70 kb apart flanking two GTP-binding protein genes, GNAI-2 and GNAT-1. In contrast, other human semaphorin gene sequences (human semaphorin III and homologues of murine semaphorins B and C) are not located on chromosome 3. Human semaphorin A(V) is translated in vitro into a 90-kDa protein, which accumulates at the endoplasmic reticulum. The human semaphorin A(V) (3.4-kb mRNA) and IV (3.9- and 2.9-kb mRNAs) genes are expressed abundantly but differentially in a variety of human neural and nonneural tissues. Human semaphorin A(V) was expressed in only 1 out of 23 SCLCs and 7 out of 16 non-SCLCs, whereas semaphorin IV was expressed in 19 out of 23 SCLCs and 13 out of 16 non-SCLCs. Mutational analysis in semaphorin A(V) revealed mutations (germ line in one case) in 3 of 40 lung cancers. Our data suggest the need to determine the function of human semaphorins A(V) and IV in nonneural tissues and their role in the pathogenesis of lung cancer.<\/jats:p>","DOI":"10.1073\/pnas.93.9.4120","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:43:20Z","timestamp":1027680200000},"page":"4120-4125","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":181,"title":["Human semaphorins A(V) and IV reside in the 3p21.3 small cell lung cancer deletion region and demonstrate distinct expression patterns."],"prefix":"10.1073","volume":"93","author":[{"given":"Y","family":"Sekido","sequence":"first","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Bader","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Latif","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Y","family":"Chen","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F M","family":"Duh","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M H","family":"Wei","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J P","family":"Albanesi","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C C","family":"Lee","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M I","family":"Lerman","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"Minna","sequence":"additional","affiliation":[{"name":"Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1996,4,30]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.93.9.4120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:04:44Z","timestamp":1649862284000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.93.9.4120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,4,30]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1996,4,30]]}},"alternative-id":["10.1073\/pnas.93.9.4120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.93.9.4120","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,4,30]]},"assertion":[{"value":"1996-04-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}