{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:00:15Z","timestamp":1781107215663,"version":"3.54.1"},"reference-count":16,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,5,27]]},"abstract":"\n Translocations affecting the chromosomal region 15q11\u201313 and various other partners are recurrent in diffuse large-cell lymphomas (DLCL). To identify the putative gene, here named\n BCL8<\/jats:italic>\n , involved in these translocations we have cloned the breakpoint region from a DLCL patient with t(14;15)(q32;q11\u201313) and the corresponding germ-line region from chromosome 15. The genomic locus on chromosome 15 is clonally rearranged in about 4% of DLCL in agreement with the frequency of 15q11\u201313 translocations. A probe derived from the\n BCL8<\/jats:italic>\n locus on chromosome 15 detected a transcript in human testis and prostate, whereas no expression was found in spleen, thymus, and blood leukocytes. Analysis of the\n BCL8<\/jats:italic>\n cDNA clones isolated from human testis cDNA library showed that the\n BCL8<\/jats:italic>\n gene generates a major transcript of 2.6 kb and a less prominent 4.5-kb species due to differential polyadenylylation. By reverse transcription\u2013PCR analysis of RNA extracted from frozen DLCL samples and lymphoma cell lines,\n BCL8<\/jats:italic>\n expression was detected in all patients carrying 15q11\u201313 abnormalities and in a fraction of randomly selected DLCL patients. These results suggest that the\n BCL8<\/jats:italic>\n gene is not normally expressed in lymphoid tissues, but its expression can be activated by chromosomal translocation or by other mechanisms in DLCL. Ectopic expression of\n BCL8<\/jats:italic>\n in a significant proportion of DLCL suggests an important role for this gene in the molecular pathogenesis of B cell lymphoma.\n <\/jats:p>","DOI":"10.1073\/pnas.94.11.5728","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:44:19Z","timestamp":1027680259000},"page":"5728-5732","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":37,"title":["BCL8<\/i>\n , a novel gene involved in translocations affecting band 15q11\u201313 in diffuse large-cell\u2009lymphoma"],"prefix":"10.1073","volume":"94","author":[{"given":"Vadim G.","family":"Dyomin","sequence":"first","affiliation":[{"name":"Cell Biology Program and the Department of Human Genetics, Memorial Sloan\u2013Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; and Division of Oncology, Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pulivarthi H.","family":"Rao","sequence":"additional","affiliation":[{"name":"Cell Biology Program and the Department of Human Genetics, Memorial Sloan\u2013Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; and Division of Oncology, Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Dalla-Favera","sequence":"additional","affiliation":[{"name":"Cell Biology Program and the Department of Human Genetics, Memorial Sloan\u2013Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; and Division of Oncology, Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S. K.","family":"Chaganti","sequence":"additional","affiliation":[{"name":"Cell Biology Program and the Department of Human Genetics, Memorial Sloan\u2013Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; and Division of Oncology, Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,5,27]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"73","volume-title":"Molecular Genetics in Cancer Diagnosis","author":"Chaganti R S K","year":"1991","unstructured":"R S K Chaganti, E A Klein Molecular Genetics in Cancer Diagnosis, ed J Cossman (Elsevier, New York), pp. 73\u2013101 (1991)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/372143a0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-109-12-939"},{"key":"e_1_3_3_4_2","first-page":"29","volume-title":"The Non-Hodgkin Lymphoma","author":"Magrath I","year":"1989","unstructured":"I Magrath The Non-Hodgkin Lymphoma, ed I Magrath (Williams & Wilkins, Baltimore), pp. 29\u201348 (1989)."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V77.5.1057.1057"},{"key":"e_1_3_3_6_2","first-page":"853","volume-title":"Hematology\/Oncology Clinics of North America: Non-Hodgkin\u2019s Lymphoma","author":"Offit K","year":"1991","unstructured":"K Offit, R S K Chaganti Hematology\/Oncology Clinics of North America: Non-Hodgkin\u2019s Lymphoma, ed J O Armitage (Saunders, Philadelphia), pp. 853\u2013869 (1991)."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199407143310202"},{"key":"e_1_3_3_8_2","volume-title":"Catalog of Chromosomal Aberrations in Cancer","author":"Mittelman F","year":"1994","unstructured":"F Mittelman Catalog of Chromosomal Aberrations in Cancer (Wiley\u2013Liss, New York, 1994)."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/3.6.853"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1471"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1993.1206"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-199203000-00005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02912.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1994.059.01.015"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/4.suppl_1.1757"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1096-163"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.11.5728","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:34:09Z","timestamp":1649860449000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.11.5728"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,5,27]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1997,5,27]]}},"alternative-id":["10.1073\/pnas.94.11.5728"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.11.5728","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,5,27]]},"assertion":[{"value":"1997-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-04-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}