{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T15:14:02Z","timestamp":1793373242301,"version":"4.5.0"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,5,27]]},"abstract":"<jats:p>The cytokines interleukin (IL)-4 and IL-13 play a critical role in inducing C\u025b germline transcripts and IgE isotype switching in human B cells. The IL-4 receptor (IL-4R) in B cells is composed of two chains, the IL-4-binding IL-4R\u03b1 chain, which is shared with the IL-13R, and the IL-2R\u03b3 (\u03b3c) chain, which is shared with IL-7R, IL-9R, and IL-15R. IL-4 induces C\u025b germline transcripts and IgE isotype switching in B cells from patients with \u03b3c chain deficiency. Induction of C\u025b germline transcripts by IL-4 in B cells that lack the \u03b3c chain may involve signaling via the IL-13R. Alternatively, the IL-4R\u03b1 chain may transduce intracellular signals that lead to C\u025b gene transcription independently of its association with other chains. We show that ligand-induced homodimerization of chimeric surface receptors consisting of the extracellular and transmembrane domains of the erythropoietin receptor and of the intracellular domain of IL-4R\u03b1 induces Janus kinase 1 (Jak1) activation, STAT6 activation, and C\u025b germline transcripts in human B cell line BJAB. Disruption of the Jak1-binding proline-rich Box1 region of IL-4R\u03b1 abolished signaling by this chimeric receptor. Furthermore, B cells transfected with a chimeric CD8\u03b1\/IL-4R\u03b1 receptor, which is expressed on the cell surface as a homodimer, constitutively expressed C\u025b germline transcripts. These results suggest that homodimerization of the IL-4R\u03b1 chain is sufficient to transduce Jak1-dependent intracellular signals that lead to IgE isotype switching.<\/jats:p>","DOI":"10.1073\/pnas.94.11.5866","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"5866-5871","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":41,"title":["Homodimerization of the human interleukin 4 receptor \u03b1 chain induces C\u025b germline transcripts in B cells in the absence of the interleukin 2 receptor \u03b3\u2009chain"],"prefix":"10.1073","volume":"94","author":[{"given":"Hiroshi","family":"Fujiwara","sequence":"first","affiliation":[{"name":"Division of Immunology, Children\u2019s Hospital, and Department of Pediatrics, Harvard Medical School, Enders 8th Floor, 300 Longwood Avenue, Boston, MA 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