{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:18:58Z","timestamp":1781104738151,"version":"3.54.1"},"reference-count":15,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,6,10]]},"abstract":"<jats:p>\n                    Mammalian selenocysteine-containing thioredoxin reductase (TR)\n isolated from HeLa cells and from human lung adenocarcinoma cells was\n separated into two major enzyme species by heparin-agarose affinity\n chromatography. The low-affinity enzyme forms that were not retained on\n heparin agarose showed strong crossreactivity in immunoblot assays with\n anti-rat liver TR polyclonal antibodies, whereas the high-affinity\n enzyme forms that were retained by the heparin column were not\n detected. Both low and high heparin-affinity enzyme forms contained\n FAD, were indistinguishable on SDS\/PAGE analysis, and exhibited\n similar catalytic activities in the NADPH-dependent DTNB\n [5,5\u2032-dithiobis(2-nitrobenzoate)] assay. The C-terminal amino acid\n sequences of\n                    <jats:sup>75<\/jats:sup>\n                    Se-labeled tryptic peptides from lung\n adenocarcinoma low- and high heparin-affinity enzyme forms were\n identical to the predicted C-terminal sequence of human placental TR.\n These two determined peptide sequences were\n -Ser-Gly-Ala-Ser-Ile-Leu-Gln-Ala-Gly-Cys-Secys-(Gly). Occurrence of the\n Se-carboxymethyl derivative of radioactive selenocysteine in the\n position corresponding to TGA in the gene confirmed that UGA is\n translated as selenocysteine. The presence of cysteine followed by a\n reactive selenocysteine residue in this C-terminal region of the\n protein may explain some of the unusual properties of the mammalian\n TRs.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.12.6138","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"6138-6141","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":33,"title":["Heparin-binding properties of selenium-containing thioredoxin reductase from HeLa cells and human lung adenocarcinoma\u2009cells"],"prefix":"10.1073","volume":"94","author":[{"given":"Song-Yuan","family":"Liu","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry, National Heart, Lung, and Blood\r Institute, National Institutes of Health, Building 3, Room 108,\r Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thressa C.","family":"Stadtman","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, National Heart, Lung, and Blood\r Institute, National Institutes of Health, Building 3, Room 108,\r Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,6,10]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00444a029"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.3.1006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)40204-3"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.6146"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00269a003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7478"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/00327487708061633"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9084(93)90131-B"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00079a025"},{"key":"e_1_3_2_10_2","first-page":"1885","volume":"38","author":"Chen C-C","year":"1978","unstructured":"C-C Chen, E C Moore, B L Borns-McCall Cancer Res 38, 1885\u20131888 (1978).","journal-title":"Cancer Res"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)01003-W"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.54.070185.001321"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00429a036"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00429a037"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)38248-6"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.12.6138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:55:33Z","timestamp":1649861733000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.12.6138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,10]]},"references-count":15,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1997,6,10]]}},"alternative-id":["10.1073\/pnas.94.12.6138"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.12.6138","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,10]]},"assertion":[{"value":"1997-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-06-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}