{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:45:57Z","timestamp":1792799157797,"version":"4.5.0"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,6,24]]},"abstract":"<jats:p>\n                    The mechanisms controlling the production of aqueous humor and the regulation of intraocular pressure are poorly understood. Here, we provide evidence that a vacuolar H\n                    <jats:sup>+<\/jats:sup>\n                    -ATPase (V-ATPase) in the ocular ciliary epithelium is a key component of this process. In intracellular pH (pH\n                    <jats:sub>i<\/jats:sub>\n                    ) measurements of isolated ciliary epithelium performed with 2\u2032,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF), the selective V-ATPase inhibitor bafilomycin A\n                    <jats:sub>1<\/jats:sub>\n                    slowed the recovery of pH\n                    <jats:sub>i<\/jats:sub>\n                    in response to acute intracellular acidification, demonstrating the presence of V-ATPase in the plasma membrane. In isolated rabbit ciliary body preparations examined under voltage-clamped conditions, bafilomycin A\n                    <jats:sub>1<\/jats:sub>\n                    produced a concentration-dependent decrease in short-circuit current, and topical application of bafilomycin A\n                    <jats:sub>1<\/jats:sub>\n                    reduced intraocular pressure in rabbits, indicating an essential role of the V-ATPase in ciliary epithelial ion transport. Immunocytochemistry utilizing antibodies specific for the B1 isoform of the V-ATPase 56-kDa subunit revealed localization of V-ATPase in both the plasma membrane and cytoplasm of the native ciliary epithelium in both rabbit and rat eye. The regional and subcellular distribution of V-ATPase in specific regions of the ciliary process was altered profoundly by isoproterenol and phorbol esters, suggesting that change in the intracellular distribution of the enzyme is a mechanism by which drugs, hormones, and neurotransmitters modify aqueous humor production.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.13.6752","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"6752-6757","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":34,"title":["Vacuolar H\n                    <sup>+<\/sup>\n                    -ATPase in ocular ciliary\u2009epithelium"],"prefix":"10.1073","volume":"94","author":[{"given":"Martin B.","family":"Wax","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences and Renal Division, Department of Medicine and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110; Department of Ophthalmology, Northwestern University Medical School, 300 East Superior Street, Chicago, IL 60611; Department of Biochemistry, Tel-Aviv University, Ramat-aviv, 69978 Tel-Aviv, Israel; and Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences and Renal Division, Department of Medicine and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110; Department of Ophthalmology, Northwestern University Medical School, 300 East Superior Street, Chicago, IL 60611; Department of Biochemistry, Tel-Aviv University, Ramat-aviv, 69978 Tel-Aviv, Israel; and Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 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Ophthalmology, Northwestern University Medical School, 300 East Superior Street, Chicago, IL 60611; Department of Biochemistry, Tel-Aviv University, Ramat-aviv, 69978 Tel-Aviv, Israel; and Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences and Renal Division, Department of Medicine and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110; Department of Ophthalmology, Northwestern University Medical School, 300 East Superior Street, Chicago, IL 60611; Department of Biochemistry, Tel-Aviv University, Ramat-aviv, 69978 Tel-Aviv, Israel; and Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 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Northwestern University Medical School, 300 East Superior Street, Chicago, IL 60611; Department of Biochemistry, Tel-Aviv University, Ramat-aviv, 69978 Tel-Aviv, Israel; and Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Gluck","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences and Renal Division, Department of Medicine and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110; Department of Ophthalmology, Northwestern University Medical School, 300 East Superior Street, Chicago, IL 60611; Department of Biochemistry, Tel-Aviv University, Ramat-aviv, 69978 Tel-Aviv, Israel; and Renal Unit, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 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