{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:44:32Z","timestamp":1781109872754,"version":"3.54.1"},"reference-count":60,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,7,8]]},"abstract":"<jats:p>\n                    Earlier studies have shown that Kaposi sarcomas contain cells infected with human herpesvirus (HHV) 6B, and in current studies we report that both AIDS-associated and classic-sporadic Kaposi sarcoma contain HHV-7 genome sequences detectable by PCR. To determine the distribution of HHV-7-infected cells relative to those infected with HHV-6, sections from paraffin-embedded tissues were allowed to react with antibodies to HHV-7 virion tegument phosphoprotein pp85 and to HHV-6B protein p101. The antibodies are specific for HHV-7 and HHV-6B, respectively, and they retained reactivity for antigens contained in formalin-fixed, paraffin-embedded tissue samples. We report that (\n                    <jats:italic>i<\/jats:italic>\n                    ) HHV-7 pp85 was present in 9 of 32 AIDS-associated Kaposi sarcomas, and in 1 of 7 classical-sporadic HIV-negative Kaposi sarcomas; (\n                    <jats:italic>ii<\/jats:italic>\n                    ) HHV-7 pp85 was detected primarily in cells bearing the CD68 marker characteristic of the monocyte\/macrophage lineage present in or surrounding the Kaposi sarcoma lesions; and (\n                    <jats:italic>iii<\/jats:italic>\n                    ) in a number of Kaposi sarcoma specimens, tumor-associated CD68\n                    <jats:sup>+<\/jats:sup>\n                    monocytes\/macrophages expressed simultaneously antigens from both HHV-7 and HHV-6B, and therefore appeared to be doubly infected with the two viruses. CD68\n                    <jats:sup>+<\/jats:sup>\n                    monocytes\/macrophages infected with HHV-7 were readily detectable in Kaposi sarcoma, but virtually absent from other normal or pathological tissues that harbor macrophages. Because all of the available data indicate that HHV-7 infects CD4\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes, these results suggest that the environment of the Kaposi sarcoma (\n                    <jats:italic>i<\/jats:italic>\n                    ) attracts circulating peripheral lymphocytes and monocytes, triggers the replication of latent viruses, and thereby increases the local concentration of viruses, (\n                    <jats:italic>ii<\/jats:italic>\n                    ) renders CD68\n                    <jats:sup>+<\/jats:sup>\n                    monocytes\/macrophages susceptible to infection with HHV-7, and (\n                    <jats:italic>iii<\/jats:italic>\n                    ) the combination of both events enables double infections of cells with both HHV-6B and HHV-7.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.14.7600","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:42:40Z","timestamp":1027680160000},"page":"7600-7605","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":53,"title":["CD68\n                    <sup>+<\/sup>\n                    cells of monocyte\/macrophage lineage in the environment of AIDS-associated and classic-sporadic Kaposi sarcoma are singly or doubly infected with human herpesviruses 7 and\u20096B"],"prefix":"10.1073","volume":"94","author":[{"given":"W.","family":"Kempf","sequence":"first","affiliation":[{"name":"Departments of Dermatology and Pathology, University Hospital, 8091 Zurich, Switzerland; 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