{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:32:10Z","timestamp":1792679530077,"version":"4.5.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,9,30]]},"abstract":"<jats:p>\n                    Spectrin (\u03b2I\u03a3\u2217) and ankyrin (Ank\n                    <jats:sub>G119<\/jats:sub>\n                    ) associate with Golgi membranes and the dynactin complex, but their role in vesicle trafficking remains uncertain. We find that the actin-binding domain and membrane-association domain 1 (MAD1) of \u03b2I spectrin together form a constitutive Golgi targeting signal in transfected MDCK cells. Expression of this signal in transfected cells disrupts the endogenous Golgi spectrin skeleton and blocks transport of \u03b1- and \u03b2-Na,K-ATPase and vesicular stomatitis virus-G protein from the endoplasmic reticulum (ER) but does not disrupt the formation of Golgi stacks, the distribution of \u03b2-COP, or the transport and surface display of E-cadherin. The Golgi spectrin skeleton is thus required for the transport of a subset of membrane proteins from the ER to the Golgi. We postulate that together with polyfunctional adapter proteins such as Ank\n                    <jats:sub>G119<\/jats:sub>\n                    , Golgi spectrin forms a docking complex that acts prior to the cis-Golgi, presumably with vesicular\u2013tubular clusters (VTCs or ERGIC), to sequester specific membrane proteins into vesicles transiting between the ER and Golgi, and subsequently (probably involving other isoforms of spectrin and ankyrin) to mediate cargo transport within the Golgi and to other membrane compartments. We hypothesize that this vesicular spectrin\u2013ankyrin adapter-protein trafficking (or tethering) system (SAATS) mediates the capture and transport of many membrane proteins and acts in conjunction with vesicle-targeting molecules to effect the efficient transport of cargo proteins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.20.10711","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"10711-10716","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":109,"title":["Na,K-ATPase transport from endoplasmic reticulum to Golgi requires the Golgi spectrin\u2013ankyrin G119 skeleton in Madin Darby canine kidney\u2009cells"],"prefix":"10.1073","volume":"94","author":[{"given":"Prasad","family":"Devarajan","sequence":"first","affiliation":[{"name":"Departments of Pathology and Pediatrics, Yale University, New Haven, CT 06520; and Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Stabach","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Pediatrics, Yale University, New Haven, CT 06520; and Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Antonietta","family":"De Matteis","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Pediatrics, Yale University, New Haven, CT 06520; and Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon S.","family":"Morrow","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Pediatrics, Yale University, New Haven, CT 06520; and Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,9,30]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5259.227"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.14.7589986"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5255.1526"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(94)90073-6"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.1.1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.3.707"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.4.819"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.6.1815"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.10.1239"},{"key":"e_1_3_3_10_2","first-page":"324a","volume":"7","author":"Devarajan P","year":"1996","unstructured":"P Devarajan, P Stabach, J S Morrow Mol Biol Cell 7, 324a, (abstr.). (1996).","journal-title":"Mol Biol Cell"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38473-X"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)30525-2"},{"key":"e_1_3_3_13_2","first-page":"1117","volume":"74","author":"Weed S A","year":"1996","unstructured":"S A Weed, P R Stabach, C E Oyer, P G Gallagher, J S Morrow Lab Invest 74, 1117\u20131129 (1996).","journal-title":"Lab Invest"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.125.5.1025"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240300107"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.106.1.67"},{"key":"e_1_3_3_17_2","first-page":"485","volume-title":"Handbook of Physiology","author":"Morrow J S","year":"1997","unstructured":"J S Morrow, D L Rimm, S P Kennedy, C D Cianci, J H Sinard, S A Weed Handbook of Physiology, eds J Hoffman, J Jamieson (Oxford Univ. Press, London), pp. 485\u2013540 (1997)."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.1.267"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)62032-6"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2818"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.7.4.631"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.2.455"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.3.893"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.130.5.1105"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.5.1751"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55045-6"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.1.19"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.4.875"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.4.895"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5325.556"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50333-7"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.51.30567"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.3.837"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.5.1059"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(89)90214-1"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41795-9"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38474-1"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(89)90529-4"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(85)90022-6"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.8.2965"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.20.10711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:02:08Z","timestamp":1649862128000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.20.10711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9,30]]},"references-count":40,"journal-issue":{"issue":"20","published-print":{"date-parts":[[1997,9,30]]}},"alternative-id":["10.1073\/pnas.94.20.10711"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.20.10711","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9,30]]},"assertion":[{"value":"1997-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-08-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-09-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}