{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:39:44Z","timestamp":1791157184726,"version":"4.1.0"},"reference-count":29,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,11,11]]},"abstract":"<jats:p>\n                    We used targeted gene disruption in mice to ablate nonmuscle myosin heavy chain B (NMHC-B), one of the two isoforms of nonmuscle myosin II present in all vertebrate cells. Approximately 65% of the\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    embryos died prior to birth, and those that were born suffered from congestive heart failure and died during the first day. No abnormalities were detected in\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    mice. The absence of NMHC-B resulted in a significant increase in the transverse diameters of the cardiac myocytes from 7.8 \u00b1 1.8 \u03bcm (right ventricle) and 7.8 \u00b1 1.3 \u03bcm (left ventricle) in\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    and\n                    <jats:italic>B<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    mice to 14.7 \u00b1 1.1 \u03bcm and 13.8 \u00b1 2.3 \u03bcm, respectively, in\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice (in both cases,\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). The increase in size of the cardiac myocytes was seen as early as embryonic day 12.5 (4.5 \u00b1 0.2 \u03bcm for\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    and\n                    <jats:italic>B<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    vs. 7.2 \u00b1 0.6 \u03bcm for\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01)). Six of seven\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    newborn mice analyzed by serial sectioning also showed structural cardiac defects, including a ventricular septal defect, an aortic root that either straddled the defect or originated from the right ventricle, and muscular obstruction to right ventricular outflow. Some of the hearts of\n                    <jats:italic>NMHC-B<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice showed evidence for up-regulation of NMHC-A protein. These studies suggest that nonmuscle myosin II-B is required for normal cardiac myocyte development and that its absence results in structural defects resembling, in part, two common human congenital heart diseases, tetralogy of Fallot and double outlet right ventricle.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.23.12407","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:44Z","timestamp":1027679504000},"page":"12407-12412","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":279,"title":["Nonmuscle myosin II-B is required for normal development of the mouse\u2009heart"],"prefix":"10.1073","volume":"94","author":[{"given":"Antonella N.","family":"Tullio","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Accili","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor J.","family":"Ferrans","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zu-Xi","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyo","family":"Takeda","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Grinberg","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiner","family":"Westphal","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette A.","family":"Preston","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Adelstein","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cardiology and Pathology Section, National Heart, Lung, and Blood Institute; and Developmental Endocrinology Branch and Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,11,11]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"1323","volume":"2","author":"Sellers J R","year":"1995","unstructured":"J R Sellers, H V Goodson Protein Profile 2, 1323\u20131423 (1995).","journal-title":"Protein Profile"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.2.659"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.3.1164"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.69.2.530"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15057.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80074-D"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.5.915"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1108"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86956-9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14533"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37123-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.12.3661"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00114503"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3576222"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.5.1499"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/382455a0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.3.675"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.11.3077"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00140325"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970280102"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970310203"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90611-F"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.16.7386"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.2.211"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.9.1019"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118920"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1096-210"},{"key":"e_1_3_2_28_2","first-page":"111","volume-title":"Molecular Biology of Cardiovascular Disease","author":"Gelb B D","year":"1997","unstructured":"B D Gelb Molecular Biology of Cardiovascular Disease, eds A R Marks, M B Taubman (Dekker, New York), pp. 111\u2013140 (1997)."},{"key":"e_1_3_2_29_2","first-page":"1","volume":"7","author":"Anderson R H","year":"1978","unstructured":"R H Anderson Prog Cardiol 7, 1\u201354 (1978).","journal-title":"Prog Cardiol"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.23.12407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:22:20Z","timestamp":1649859740000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.23.12407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11,11]]},"references-count":29,"journal-issue":{"issue":"23","published-print":{"date-parts":[[1997,11,11]]}},"alternative-id":["10.1073\/pnas.94.23.12407"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.23.12407","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11,11]]},"assertion":[{"value":"1997-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-11-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}