{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:20:43Z","timestamp":1790864443165,"version":"4.1.0"},"reference-count":21,"publisher":"National Academy of Sciences","issue":"25","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,12,9]]},"abstract":"<jats:p>\n                    Human epithelial kidney cells (HEK) were prepared to coexpress \u03b11A, \u03b12\u03b4 with different \u03b2 calcium channel subunits and green fluorescence protein. To compare the calcium currents observed in these cells with the native neuronal currents, electrophysiological and pharmacological tools were used conjointly. Whole-cell current recordings of human epithelial kidney \u03b11A-transfected cells showed small inactivating currents in 80 mM Ba\n                    <jats:sup>2+<\/jats:sup>\n                    that were relatively insensitive to calcium blockers. Coexpression of \u03b11A, \u03b2Ib, and \u03b12\u03b4 produced a robust inactivating current detected in 10 mM Ba\n                    <jats:sup>2+<\/jats:sup>\n                    , reversibly blockable with low concentration of \u03c9-agatoxin IVA (\u03c9-Aga IVA) or synthetic funnel-web spider toxin (sFTX). Barium currents were also supported by \u03b11A, \u03b22a, \u03b12\u03b4 subunits, which demonstrated the slowest inactivation and were relatively insensitive to \u03c9-Aga IVA and sFTX. Coexpression of \u03b23 with the same combination as above produced inactivating currents also insensitive to low concentration of \u03c9-Aga IVA and sFTX. These data indicate that the combination \u03b11A, \u03b2Ib, \u03b12\u03b4 best resembles P-type channels given the rate of inactivation and the high sensitivity to \u03c9-Aga IVA and sFTX. More importantly, the specificity of the channel blocker is highly influenced by the \u03b2 subunit associated with the \u03b11A subunit.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.25.14042","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:42:40Z","timestamp":1027680160000},"page":"14042-14047","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":53,"title":["\u03b2 subunits influence the biophysical and pharmacological differences between P- and Q-type calcium currents  expressed in a mammalian cell\u2009line"],"prefix":"10.1073","volume":"94","author":[{"given":"Herman","family":"Moreno","sequence":"first","affiliation":[{"name":"Departments of Physiology and Neuroscience and Biochemistry, New York University Medical Center, 550 First Avenue, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo","family":"Rudy","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Neuroscience and Biochemistry, New York University Medical Center, 550 First Avenue, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Llin\u00e1s","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Neuroscience and Biochemistry, New York University Medical Center, 550 First Avenue, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,12,9]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.5.1689"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90223-Z"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-04-02995.1995"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(93)90003-L"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10576"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/350398a0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.17.030194.002151"},{"key":"e_1_3_3_8_2","first-page":"1183","volume":"12","author":"Isom L","year":"1994","unstructured":"L Isom, K DeJongh, W Catterall Nature (London) 12, 1183\u20131194 (1994).","journal-title":"Nature (London)"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp021029"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90185-T"},{"key":"e_1_3_3_11_2","unstructured":"Gillard S. 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