{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:58:49Z","timestamp":1792457929360,"version":"4.5.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,12,23]]},"abstract":"<jats:p>\n                    The first Zn(II)-translocating P-type ATPase has been identified as the product of\n                    <jats:italic>o732<\/jats:italic>\n                    , a potential gene identified in the sequencing of the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    genome. This gene, termed\n                    <jats:italic>zntA<\/jats:italic>\n                    , was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain exhibited hypersensitivity to zinc and cadmium salts but not salts of other metals, suggesting a role in zinc homeostasis in\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Everted membrane vesicles from a wild-type strain accumulated\n                    <jats:sup>65<\/jats:sup>\n                    Zn(II) and\n                    <jats:sup>109<\/jats:sup>\n                    Cd(II) by using ATP as an energy source. Transport was sensitive to vanadate, an inhibitor of P-type ATPases. Membrane vesicles from the\n                    <jats:italic>zntA<\/jats:italic>\n                    \u2237\n                    <jats:italic>kan<\/jats:italic>\n                    strain did not accumulate those metal ions. Both the sensitive phenotype and transport defect of the mutant were complemented by expression of\n                    <jats:italic>zntA<\/jats:italic>\n                    on a plasmid.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.26.14326","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"14326-14331","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":379,"title":["The\n                    <i>zntA<\/i>\n                    gene of\n                    <i>Escherichia coli<\/i>\n                    encodes a Zn(II)-translocating P-type\u2009ATPase"],"prefix":"10.1073","volume":"94","author":[{"given":"Christopher","family":"Rensing","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharati","family":"Mitra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry P.","family":"Rosen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,12,23]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"245","volume-title":"Metals and Micro-organisms","author":"Hughes M N","year":"1989","unstructured":"M N Hughes, R K Poole Metals and Micro-organisms (Chapman & Hall, London), pp. 245\u2013248 (1989)."},{"key":"e_1_3_3_2_2","first-page":"259","volume":"73","author":"Vallee B L","year":"1995","unstructured":"B L Vallee, D S Auld Experientia 73, 259\u2013277 (1995).","journal-title":"Experientia"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8342038"},{"key":"e_1_3_3_4_2","first-page":"63","volume":"52","author":"Palmiter R D","year":"1987","unstructured":"R D Palmiter Experientia 52, 63\u201380 (1987).","journal-title":"Experientia"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01109.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07042.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00527.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14934"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.10.2707-2712.1995"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.6871-6879.1997"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00048a001"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(96)20016-7"},{"key":"e_1_3_3_13_2","first-page":"1091","volume-title":"Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology","author":"Silver S","year":"1996","unstructured":"S Silver Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, eds F C Neidhardt, R Curtiss, JL Ingraham, E C C Lin, K B Low, B Magasanik, W S Reznikoff, M Riley, M Schaechter, H E Umbarger (Am. 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