{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:15:03Z","timestamp":1781108103359,"version":"3.54.1"},"reference-count":60,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,2,18]]},"abstract":"<jats:p>\n                    In a patient with progressing metastatic melanoma, we showed that the same autologous tumor-cytolytic CD8\n                    <jats:sup>+<\/jats:sup>\n                    tumor infiltrating lymphocyte (TIL) clone accumulated in two separate metastatic sites. This clone, which represented three of eight independently derived clones from a tumor deposit on the skin of the abdomen, also represented two of eight clones derived from a skin lesion on the shoulder. This clone could be identified by its use of a unique TCRBV2-nD1n-J1S6 sequence, and could also be detected by single-stranded conformational polymorphism (SSCP) as the dominant TCRBV2-expressing clone among CD8\n                    <jats:sup>+<\/jats:sup>\n                    TILs propagated from both shoulder and abdominal lesions. Using SSCP analysis, we also demonstrated that this clone was dominant in the fresh tumor tissue and in all TILs in which CD8\n                    <jats:sup>+<\/jats:sup>\n                    were strongly represented, including several separate but parallel cultures. The SSCP pattern for this clone was not apparent among CD4\n                    <jats:sup>+<\/jats:sup>\n                    TILs or CD8\n                    <jats:sup>+<\/jats:sup>\n                    peripheral blood mononuclear cells. The SSCP analysis of the tumor tissue prior to\n                    <jats:italic>in vitro<\/jats:italic>\n                    culture is an indication that the selection for this anti-tumor cytotoxic T cell clone was a reflection of its\n                    <jats:italic>in vivo<\/jats:italic>\n                    accumulation. Thus, we provide evidence that melanomas are immunogenic and able to select for cytotoxic anti-tumor-specific TIL clones that are expanded\n                    <jats:italic>in vivo<\/jats:italic>\n                    and can circulate to accumulate in different tumor sites. However, because these clones were isolated from progressing tumor metastases, the accumulation of these specific cytotoxic T cells was not sufficient to contain tumor growth.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.4.1378","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:43:20Z","timestamp":1027680200000},"page":"1378-1383","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":28,"title":["<i>In vivo<\/i>\n                    accumulation of the same anti-melanoma T cell clone in two different metastatic\u2009sites"],"prefix":"10.1073","volume":"94","author":[{"given":"Makoto","family":"Hishii","sequence":"first","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Andrews","sequence":"additional","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenora\u2009A.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnson\u2009T.","family":"Wong","sequence":"additional","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Pandolfi","sequence":"additional","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter\u2009J.","family":"van\u2009den\u2009Elsen","sequence":"additional","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James\u2009T.","family":"Kurnick","sequence":"additional","affiliation":[{"name":"Pathology Research Laboratory, Massachusetts General Hospital, Charlestown, MA 02129; Chair of Semeiotica Medica, Catholic University, Rome, Italy 00168; and Department of Immunohematology and Blood Bank, Leiden University Hospital, 2300 RC, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,2,18]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.6.1961"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.4.1419"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.4.1231"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5674"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(86)90247-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00049412"},{"key":"e_1_3_2_7_2","first-page":"1292","volume":"54","author":"Caignard A","year":"1994","unstructured":"A Caignard, P Dietrich, V Morand, A Lim, C Pannetier, A Leridant, T Hercend, J Even, P Kourilsky, F Triebel Cancer Res 54, 1292\u20131297 (1994).","journal-title":"Cancer Res"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200417"},{"key":"e_1_3_2_9_2","first-page":"1919","volume":"11","author":"Ioannides C","year":"1991","unstructured":"C Ioannides, R Freedman Anticancer Res 11, 1919\u20131925 (1991).","journal-title":"Anticancer Res"},{"key":"e_1_3_2_10_2","first-page":"3569","volume":"53","author":"Mackensen A","year":"1993","unstructured":"A Mackensen, L Ferradini, G Carcelain, F Triebel, F Faure, S Viel, T Hercend Cancer Res 53, 3569\u20133573 (1993).","journal-title":"Cancer Res"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"4004","DOI":"10.4049\/jimmunol.138.11.4004","volume":"138","author":"Miescher S","year":"1987","unstructured":"S Miescher, T Whiteside, L Moretta, V VonFliedner J Immunol 138, 4004\u20134011 (1987).","journal-title":"J Immunol"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1994.tb06028.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"5472","DOI":"10.4049\/jimmunol.151.10.5472","volume":"151","author":"Peoples G","year":"1993","unstructured":"G Peoples, M Davey, P Goedegebuure, D Schoof, T Eberlein J Immunol 151, 5472\u20135480 (1993).","journal-title":"J Immunol"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198812223192527"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.4049\/jimmunol.143.10.3404","volume":"143","author":"Wong J T","year":"1989","unstructured":"J T Wong, C E Pinto, J D Gifford, J T Kurnick, R L Kradin J Immunol 143, 3404\u20133411 (1989).","journal-title":"J Immunol"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01246677"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.002005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.9.3515"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240340"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.3.1005"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"5944","DOI":"10.4049\/jimmunol.154.11.5944","volume":"154","author":"Robbins P","year":"1995","unstructured":"P Robbins, M el-Gamil, Y Li, S Topalian, L Rivoltini, K Sakaguchi, E Appella, Y Kawakami, S Rosenberg J Immunol 154, 5944\u20135950 (1995).","journal-title":"J Immunol"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.799"},{"key":"e_1_3_2_23_2","first-page":"3164","volume":"51","author":"Pandolfi F","year":"1991","unstructured":"F Pandolfi, L A Boyle, L Trentin, J T Kurnick, K J Isselbacher, S Gattoni Celli Cancer Res 51, 3164\u20133170 (1991).","journal-title":"Cancer Res"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80033-6"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80059-5"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19920501)69:9<2379::AID-CNCR2820690928>3.0.CO;2-W"},{"key":"e_1_3_2_27_2","first-page":"4649","volume":"52","author":"Ferradini L","year":"1992","unstructured":"L Ferradini, S Roman-Roman, J Azocar, M Avril, S Viel, F Triebel, T Hercend Cancer Res 52, 4649\u20134654 (1992).","journal-title":"Cancer Res"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01756599"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2382141"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.4049\/jimmunol.153.6.2807","volume":"153","author":"Puisieux I","year":"1994","unstructured":"I Puisieux, J Even, C Pannetier, F Jotereau, M Favrot, P Kourilsky J Immunol 153, 2807\u20132818 (1994).","journal-title":"J Immunol"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2829"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(91)90114-P"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910560115"},{"key":"e_1_3_2_34_2","first-page":"1195","volume":"46","author":"Tomita S","year":"1987","unstructured":"S Tomita, M T Lotze, S A Rosenberg Fed Proc 46, 1195, (abstr.). (1987).","journal-title":"Fed Proc"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117116"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(89)90127-X"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.2820690517"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.4049\/jimmunol.122.4.1255","volume":"122","author":"Kurnick J","year":"1978","unstructured":"J Kurnick, K Gronvik, A Kimura, J Lindblom, V Skoog, O Sjoberg, H Wigzell J Immunol 122, 1255\u20131260 (1978).","journal-title":"J Immunol"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.4049\/jimmunol.126.4.1307","volume":"126","author":"Kurnick J","year":"1981","unstructured":"J Kurnick, A Hayward, P Altevogt J Immunol 126, 1307\u20131311 (1981).","journal-title":"J Immunol"},{"key":"e_1_3_2_40_2","first-page":"394","volume":"19","author":"Stegagno M","year":"1987","unstructured":"M Stegagno, L Boyle, F Preffer, C Leary, R Colvin, A Cosimi, J Kurnick Transplant Proc 19, 394\u2013397 (1987).","journal-title":"Transplant Proc"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(93)90507-W"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.13.4028"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/1050-3862(92)90001-L"},{"key":"e_1_3_2_45_2","first-page":"490","volume":"17","author":"Vidal-Puig A","year":"1994","unstructured":"A Vidal-Puig, D Moller BioTechniques 17, 490\u2013496 (1994).","journal-title":"BioTechniques"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.2.789.bloodjournal872789"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.159.1.57"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(91)90086-O"},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.4049\/jimmunol.144.11.4487","volume":"144","author":"Topalian S","year":"1990","unstructured":"S Topalian, A Kasid, S Rosenberg J Immunol 144, 4487\u20134489 (1990).","journal-title":"J Immunol"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.8.2804"},{"key":"e_1_3_2_51_2","first-page":"1627","volume":"54","author":"Oaks M","year":"1994","unstructured":"M Oaks, J J Hanson, D O\u2019Malley Cancer Res 54, 1627\u20131629 (1994).","journal-title":"Cancer Res"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6060(05)80054-6"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910470413"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90006-8"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)91609-7"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01533382"},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.4049\/jimmunol.146.10.3674","volume":"146","author":"Schwartzentruber D","year":"1991","unstructured":"D Schwartzentruber, S Topalian, M Mancini, S Rosenberg J Immunol 146, 3674\u20133681 (1991).","journal-title":"J Immunol"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.10.1605"},{"key":"e_1_3_2_59_2","first-page":"208","volume":"129","author":"Cohen P","year":"1987","unstructured":"P Cohen, M Lotze, J Roberts, S Rosenberg, E Jaffee Am J Pathol 129, 208\u2013216 (1987).","journal-title":"Am J Pathol"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370715"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.4.1378","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T08:30:59Z","timestamp":1682152259000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.4.1378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,18]]},"references-count":60,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,2,18]]}},"alternative-id":["10.1073\/pnas.94.4.1378"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.4.1378","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2,18]]},"assertion":[{"value":"1996-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1996-11-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-02-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}