{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:59:44Z","timestamp":1781107184880,"version":"3.54.1"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,3,18]]},"abstract":"<jats:p>\n                    The development of T cells within the thymus is largely dependent on intact cortical and medullary epithelial cells. However, it has been reported that positive selection of natural killer antigen 1.1\n                    <jats:sup>+<\/jats:sup>\n                    (NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    ) T cell antigen receptor (TCR)-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    thymocytes recently identified among CD4\n                    <jats:sup>+<\/jats:sup>\n                    8\n                    <jats:sup>\u2212<\/jats:sup>\n                    and CD4\n                    <jats:sup>\u2212<\/jats:sup>\n                    8\n                    <jats:sup>\u2212<\/jats:sup>\n                    subpopulations is attributable to major histocompatibility complex class Ib ligands expressed on bone marrow (BM)-derived components in the thymus. In the present study, we investigated generation of NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    TCR-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    cells in the thymus of the\n                    <jats:italic>aly<\/jats:italic>\n                    \/\n                    <jats:italic>aly<\/jats:italic>\n                    mouse which lacks lymph nodes and Peyer\u2019s patches and shows abnormalities of thymic and splenic structure. We found that the proportion of the NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    TCR-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    thymocytes was extremely low in these mice as compared with\n                    <jats:italic>aly<\/jats:italic>\n                    \/+ and normal C57BL\/6 mice. Thymic reconstitution by BM cells from\n                    <jats:italic>aly<\/jats:italic>\n                    \/+ mice that possess a normal population of NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    TCR-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    cell population did not restore the NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    TCR-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    cell population in the thymus of lethally irradiated\n                    <jats:italic>aly<\/jats:italic>\n                    \/\n                    <jats:italic>aly<\/jats:italic>\n                    mouse. When deoxyguanosine-treated fetal thymi from (B6 \u00d7 B10.G)F\n                    <jats:sub>1<\/jats:sub>\n                    mice were transplanted to\n                    <jats:italic>aly<\/jats:italic>\n                    \/\n                    <jats:italic>aly<\/jats:italic>\n                    mice that had been thymectomized and reconstituted with BM cells of\n                    <jats:italic>aly<\/jats:italic>\n                    \/\n                    <jats:italic>aly<\/jats:italic>\n                    mice, normal proportions of the NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    TCR-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    thymocytes were present in the thymus grafts. These findings demonstrate that the development of NK1.1\n                    <jats:sup>+<\/jats:sup>\n                    TCR-\u03b1\/\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    thymocytes is accomplished under the influence not only of BM-derived components, but also of irradiation-resistant or deoxyguanosine-resistant components and an intact microenvironment of the thymus.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.6.2472","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"2472-2477","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":29,"title":["Generation of NK1.1\n                    <sup>+<\/sup>\n                    T cell antigen receptor \u03b1\/\u03b2\n                    <sup>+<\/sup>\n                    thymocytes associated with intact thymic\u2009structure"],"prefix":"10.1073","volume":"94","author":[{"given":"Ken-ichi","family":"Nakagawa","sequence":"first","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuya","family":"Iwabuchi","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumasa","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Ato","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaharu","family":"Kajiwara","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Nishihori","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chikako","family":"Iwabuchi","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, School of Medicine, Hokkaido University, Sapporo 060, Japan; and All Children\u2019s Hospital, University of South Florida, St. Petersburg, FL 33701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ishikura","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, First Department of Pathology, 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