{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:59:11Z","timestamp":1791295151978,"version":"4.1.0"},"reference-count":28,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,4]]},"abstract":"<jats:p>\n                    The elucidation of the mechanisms of antigen presentation by major histocompatibility complex class I molecules has stimulated the search for nonreplicative vectors that could deliver CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell epitopes to the cytosol of antigen-presenting cells to trigger the activation of specific cytotoxic T lymphocytes (CTLs)\n                    <jats:italic>in vivo<\/jats:italic>\n                    . In the present study, we investigated the potential ability of an invasive adenylate cyclase toxin from\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    , carrying a CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell epitope from the nucleoprotein of lymphocytic choriomeningitis virus (LCMV), to stimulate protective anti-viral immunity. Mice immunized with this recombinant toxin developed strong CTL responses against LCMV-infected target cells. Moreover, these mice were protected against an intracerebral challenge with a virulent strain of LCMV that killed all nonimmunized mice within 7 days. This protection was abolished after\n                    <jats:italic>in vivo<\/jats:italic>\n                    elimination of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. A mutant toxin devoid of adenylate cyclase activity (i.e., cAMP synthesizing activity) was constructed by insertion of a dipeptide into the catalytic site of the molecule. This genetically detoxified invasive toxin carrying the LCMV epitope stimulated a strong CTL response against both peptide-coated and virus-infected target cells, and mice immunized with this molecule were fully protected against a lethal intracerebral LCMV challenge. To our knowledge, this study represents the first demonstration that a genetically detoxified bacterial toxin carrying a viral CTL epitope can stimulate efficient protective immunity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.7.3314","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"3314-3319","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":79,"title":["Anti-viral protection conferred by recombinant adenylate cyclase toxins from\n                    <i>Bordetella pertussis<\/i>\n                    carrying a CD8\n                    <sup>+<\/sup>\n                    T cell epitope from lymphocytic choriomeningitis\u2009virus"],"prefix":"10.1073","volume":"94","author":[{"given":"M. F.","family":"Saron","sequence":"first","affiliation":[{"name":"Unit\u00e9 d\u2019Histopathologie-Virologie Exp\u00e9rimentale, Unit\u00e9 de Biologie des R\u00e9gulations Immunitaires, and Unit\u00e9 de Biochimie des R\u00e9gulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Fayolle","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d\u2019Histopathologie-Virologie Exp\u00e9rimentale, Unit\u00e9 de Biologie des R\u00e9gulations Immunitaires, and Unit\u00e9 de Biochimie des R\u00e9gulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sebo","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d\u2019Histopathologie-Virologie Exp\u00e9rimentale, Unit\u00e9 de Biologie des R\u00e9gulations Immunitaires, and Unit\u00e9 de Biochimie des R\u00e9gulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Ladant","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d\u2019Histopathologie-Virologie Exp\u00e9rimentale, Unit\u00e9 de Biologie des R\u00e9gulations Immunitaires, and Unit\u00e9 de Biochimie des R\u00e9gulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ullmann","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d\u2019Histopathologie-Virologie Exp\u00e9rimentale, Unit\u00e9 de Biologie des R\u00e9gulations Immunitaires, and Unit\u00e9 de Biochimie des R\u00e9gulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Leclerc","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d\u2019Histopathologie-Virologie Exp\u00e9rimentale, Unit\u00e9 de Biologie des R\u00e9gulations Immunitaires, and Unit\u00e9 de Biochimie des R\u00e9gulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,4]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.154.2.225"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830130904"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.3.723"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.51.3.682-686.1984"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb36343.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90336-0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.10.3851-3857.1995"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"4697","DOI":"10.4049\/jimmunol.156.12.4697","volume":"156","author":"Fayolle C","year":"1996","unstructured":"C Fayolle, P Sebo, D Ladant, A Ullmann, C Leclerc J Immunol 156, 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