{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:27:13Z","timestamp":1781105233137,"version":"3.54.1"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,4,15]]},"abstract":"<jats:p>\n                    The Finkel\u2013Biskis\u2013Jinkins murine sarcoma virus, which carries v-\n                    <jats:italic>fos<\/jats:italic>\n                    , induces osteosarcomas, whereas high-level expression of exogenous c-\n                    <jats:italic>fos<\/jats:italic>\n                    in transgenic and chimeric mice leads to postnatal development of osteogenic and chondrogenic tumors, respectively. To test whether such target cell specificity of an oncogene can be detected even in early development, we induced ectopic expression of\n                    <jats:italic>fos<\/jats:italic>\n                    in chicken limb buds by microinjecting replication-competent retrovirus into the presumptive leg field of stage 10 embryos. This caused cartilage truncation of all the long bones of the injected leg, which was mainly attributable to chondrodysplasia due to severe retardation of differentiation of the proliferating chondrocytes into mature or hypertrophic chondrocytes, as well as a slight delay in precartilagenous condensation. Expression of genes for all the other known members of chicken AP-1, which include such transforming genes as c-\n                    <jats:italic>jun<\/jats:italic>\n                    and\n                    <jats:italic>fra<\/jats:italic>\n                    -2, however, caused no macroscopic abnormalities in limb formation, indicating a specific function of Fos proteins in embryonic endochondral bone differentiation. The extent of truncation was stronger with v-Fos than with c-Fos, and comparative analysis of these proteins, as well as v-Fos mutants, revealed that strong transforming activity of Fos protein is necessary to cause dysplasia, suggesting that common molecular mechanisms are involved in both embryonic chondrodysplasia and bone tumor formation in postnatal mice.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.8.3994","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"3994-3999","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":21,"title":["Chondrocytes as a specific target of ectopic Fos expression in\u2009early\u2009development"],"prefix":"10.1073","volume":"94","author":[{"given":"Hirotaka","family":"Watanabe","sequence":"first","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Saitoh","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kameda","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Murakami","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Niikura","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Okazaki","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Morishita","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Mori","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Yokouchi","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Kuroiwa","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Iba","sequence":"additional","affiliation":[{"name":"Departments of Gene Regulation and Pathology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan; and Department of Molecular Biology, School of Science, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-01, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,4,15]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90219-8"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.44.2.674-682.1982"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.151.3711.698"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.8.5.2063-2069.1988"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.9.3619"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03441.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90024-4"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.20.5537"},{"key":"e_1_3_3_9_2","first-page":"121","volume":"2","author":"Iba H","year":"1988","unstructured":"H Iba, Y Shindo, H Nisina, T Yoshida Oncogene Res 2, 121\u2013133 (1988).","journal-title":"Oncogene Res"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.10.1677"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1991.tb01746.x"},{"key":"e_1_3_3_12_2","first-page":"1541","volume":"5","author":"Castellazzi M","year":"1990","unstructured":"M Castellazzi, J P Dangy, F F Mechta, S I Hirai, M Yaniv, J Samarut, A Lassailly, G Burn Oncogene 5, 1541\u20131547 (1990).","journal-title":"Oncogene"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.6.3527-3535.1994"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9369"},{"key":"e_1_3_3_15_2","first-page":"989","volume":"5","author":"Wu J-X","year":"1990","unstructured":"J-X Wu, J M Carpenter, C Gresens, R Keh, H Niman, J W S Morris, D Mercola Oncogene 5, 989\u20131000 (1990).","journal-title":"Oncogene"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/325412a0"},{"key":"e_1_3_3_17_2","first-page":"861","volume":"4","author":"R\u00fcther U","year":"1989","unstructured":"U R\u00fcther, D Komitowski, F R Schubert, E F Wagner Oncogene 4, 861\u2013865 (1989).","journal-title":"Oncogene"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.3.685"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07783.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90343-7"},{"key":"e_1_3_3_21_2","first-page":"79","volume":"5","author":"Yoshida T","year":"1989","unstructured":"T Yoshida, Y Shindo, K Ohta, H Iba Oncogene Res 5, 79\u201389 (1989).","journal-title":"Oncogene Res"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.6.5.1545-1551.1986"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.14.4424"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.12.3733-3740.1987"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.6.2350"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.20.2509"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.107.1.23"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(91)90497-I"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1209"},{"key":"e_1_3_3_30_2","first-page":"156","volume":"162","author":"Piotr C","year":"1987","unstructured":"C Piotr, N Sacchi Anal Biochem 162, 156\u2013159 (1987).","journal-title":"Anal Biochem"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.10.4207"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)89212-8"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.12.4012-4018.1987"},{"key":"e_1_3_3_34_2","first-page":"659","volume":"3","author":"Nishimura T","year":"1988","unstructured":"T Nishimura, P K Vogt Oncogene 3, 659\u2013663 (1988).","journal-title":"Oncogene"},{"key":"e_1_3_3_35_2","first-page":"1623","volume":"6","author":"Hartl M","year":"1991","unstructured":"M Hartl, J T Hutchins, P K Vogt Oncogene 6, 1623\u20131631 (1991).","journal-title":"Oncogene"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.1.217"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/328711a0"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.8.5.2251-2256.1988"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/379262a0"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/379534a0"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.8.3994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:33:00Z","timestamp":1649863980000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.8.3994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,4,15]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1997,4,15]]}},"alternative-id":["10.1073\/pnas.94.8.3994"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.8.3994","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,4,15]]},"assertion":[{"value":"1996-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-01-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}