{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:38:56Z","timestamp":1781105936404,"version":"3.54.1"},"reference-count":70,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,4,29]]},"abstract":"<jats:p>\n                    Deletions and other genome rearrangements are associated with carcinogenesis and inheritable diseases. The pink-eyed unstable (\n                    <jats:italic>p<\/jats:italic>\n                    <jats:sup>un<\/jats:sup>\n                    ) mutation in the mouse is caused by duplication of a 70-kb internal fragment of the\n                    <jats:italic>p<\/jats:italic>\n                    gene. Spontaneous reversion events in homozygous\n                    <jats:italic>p<\/jats:italic>\n                    <jats:sup>un<\/jats:sup>\n                    \/\n                    <jats:italic>p<\/jats:italic>\n                    <jats:sup>un<\/jats:sup>\n                    mice occur through deletion of a duplicated sequence. Reversion events in premelanocytes in the mouse embryo detected as black spots on the gray fur of the offspring were inducible by the carcinogen x-rays, ethyl methanesulfonate, methyl methanesulfonate, ethyl nitrosourea, benzo[\n                    <jats:italic>a<\/jats:italic>\n                    ]pyrene, trichloroethylene, benzene, and sodium arsenate. The latter three carcinogens are not detectable with several\n                    <jats:italic>in vitro<\/jats:italic>\n                    or\n                    <jats:italic>in vivo<\/jats:italic>\n                    mutagenesis assays. We studied the molecular mechanism of the carcinogen-induced reversion events by cDNA analysis using reverse transcriptase\u2013PCR method and identified the induced reversion events as deletions. DNA deletion assays may be sensitive indicators for carcinogen exposure.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.9.4576","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"4576-4581","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":62,"title":["Carcinogens induce reversion of the mouse pink-eyed unstable\u2009mutation"],"prefix":"10.1073","volume":"94","author":[{"given":"Robert H.","family":"Schiestl","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Toxicology, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Aubrecht","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Toxicology, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 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