{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:04:45Z","timestamp":1781964285918,"version":"3.54.5"},"reference-count":23,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,4,29]]},"abstract":"<jats:p>\n                    Specificity in the immune system is dictated and regulated by specific recognition of peptide\/major histocompatibility complex (MHC) complexes by the T cell receptor. Such peptide\/MHC complexes are a desirable target for novel approaches in immunotherapy because of their highly restricted fine specificity. Recently, phage display was used to isolate an antibody that has T cell receptor-like specificity. It recognizes mouse MHC class I H-2K\n                    <jats:sup>k<\/jats:sup>\n                    molecules complexed with a H-2K\n                    <jats:sup>k<\/jats:sup>\n                    -restricted influenza virus-derived hemagglutinin peptide (Ha\n                    <jats:sub>255\u2013262<\/jats:sub>\n                    ) but does not bind to class I H-2K\n                    <jats:sup>k<\/jats:sup>\n                    alone, peptide alone, or H-2K\n                    <jats:sup>k<\/jats:sup>\n                    complexed with other peptides. We have used this antibody to make a recombinant antibody\u2013toxin fusion protein (immunotoxin) and show herein that it specifically kills antigen-presenting cells in a peptide-dependent manner and with T cell receptor-like specificity. We find a striking correlation between the fine specificity of binding of the antibody and the cytotoxic activity of the recombinant immunotoxin. We also show specific killing of influenza virus-infected target cells. The results suggest that it should be possible to develop novel immunotherapeutic strategies against human cancer by making recombinant antibodies that will recognize cancer-related peptides complexed with MHC class I molecules on the surface of cancer cells and using these to deliver toxins, radioisotopes, or cytotoxic drugs to the cancer cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.9.4631","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:39Z","timestamp":1027679499000},"page":"4631-4636","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":26,"title":["Peptide-specific killing of antigen-presenting cells by a recombinant antibody\u2013toxin fusion protein targeted to major histocompatibility complex\/peptide class I complexes with T cell receptor-like specificity"],"prefix":"10.1073","volume":"94","author":[{"given":"Yoram","family":"Reiter","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, Building 37, Room 4E16, National Institutes of Health, Bethesda, MD 20892-4255; Department of Biological Sciences, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark; and Department of Clinical Immunology, National University Hospital, Jagensvej 20, DK-2200 Copenhagen N, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina","family":"Di Carlo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, Building 37, Room 4E16, National Institutes of Health, Bethesda, MD 20892-4255; Department of Biological Sciences, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark; and Department of Clinical Immunology, National University Hospital, Jagensvej 20, DK-2200 Copenhagen N, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Fugger","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, Building 37, Room 4E16, National Institutes of Health, Bethesda, MD 20892-4255; Department of Biological Sciences, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark; and Department of Clinical Immunology, National University Hospital, Jagensvej 20, DK-2200 Copenhagen N, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Engberg","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, Building 37, Room 4E16, National Institutes of Health, Bethesda, MD 20892-4255; Department of Biological Sciences, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark; and Department of Clinical Immunology, National University Hospital, Jagensvej 20, DK-2200 Copenhagen N, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Pastan","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, Building 37, Room 4E16, National Institutes of Health, Bethesda, MD 20892-4255; Department of Biological Sciences, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark; and Department of Clinical Immunology, National University Hospital, Jagensvej 20, DK-2200 Copenhagen N, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,4,29]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.47.1.481"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2264"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2253"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.5.1820"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.19.10338"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1683495"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.61.070192.001555"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.19.8616"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1096-1239"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/346476a0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240625"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7513441"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1840703"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.3.921"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.489"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.9.3515"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.14.6458"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.347"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1323877"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90171-6"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/329512a0"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.002245"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0895-837"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.9.4631","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T16:00:56Z","timestamp":1649865656000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.9.4631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,4,29]]},"references-count":23,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1997,4,29]]}},"alternative-id":["10.1073\/pnas.94.9.4631"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.9.4631","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,4,29]]},"assertion":[{"value":"1996-12-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-04-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}