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We investigated the effects on human vision of a mutation in the RK gene causing Oguchi disease, a recessively inherited retinopathy.\n                    <jats:italic>In vitro<\/jats:italic>\n                    experiments demonstrated that the mutation, a deletion of exon 5, abolishes the enzymatic activity of RK and is likely a null. Both a homozygote and heterozygote with this RK mutation had recovery phase abnormalities of rod-isolated photoresponses by electroretinography (ERG); photoactivation was normal. Kinetics of rod bleaching adaptation by psychophysics were dramatically slowed in the homozygote but normal final thresholds were attained. Light adaptation was normal at low backgrounds but became abnormal at higher backgrounds. A slight slowing of cone deactivation kinetics in the homozygote was detected by ERG. Cone bleaching adaptation and background adaptation were normal. In this human\n                    <jats:italic>in vivo<\/jats:italic>\n                    condition without a functional RK and probable lack of phosphorylation and arrestin binding to activated rhodopsin, reduction of photolyzed chromophore and regeneration processes with 11-\n                    <jats:italic>cis<\/jats:italic>\n                    -retinal probably constitute the sole pathway for recovery of rod sensitivity. The role of RK in rods would thus be to accelerate inactivation of activated rhodopsin molecules that in concert with regeneration leads to the normal rate of recovery of sensitivity. Cones may rely mainly on regeneration for the inactivation of photolyzed visual pigment, but RK also contributes to cone recovery.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.1.328","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:11Z","timestamp":1027680011000},"page":"328-333","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":131,"title":["Null mutation in the rhodopsin kinase gene slows recovery kinetics of rod and cone phototransduction in\u2009man"],"prefix":"10.1073","volume":"95","author":[{"given":"Artur V.","family":"Cideciyan","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA 19104; Departments of Ophthalmology and Pharmacology, University of Washington, Seattle, WA 98195; and Departments of Ophthalmology and Biochemistry, State University of New York, Buffalo, NY 14215"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA 19104; 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