{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:03:55Z","timestamp":1781103835301,"version":"3.54.1"},"reference-count":35,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,6,9]]},"abstract":"<jats:p>\n                    Purines can modify ciliary epithelial secretion of aqueous humor into the eye. The source of the purinergic agonists acting in the ciliary epithelium, as in many epithelial tissues, is unknown. We found that the fluorescent ATP marker quinacrine stained rabbit and bovine ciliary epithelia but not the nerve fibers in the ciliary bodies. Cultured bovine pigmented and nonpigmented ciliary epithelial cells also stained intensely when incubated with quinacrine. Hypotonic stimulation of cultured epithelial cells increased the extracellular ATP concentration by 3-fold; this measurement underestimates actual release as the cells also displayed ecto-ATPase activity. The hypotonically triggered increase in ATP was inhibited by the Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    -channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) in both cell types. In contrast, the P-glycoprotein inhibitors tamoxifen and verapamil and the cystic fibrosis transmembrane conductance regulator (CFTR) blockers glybenclamide and diphenylamine-2-carboxylate did not affect ATP release from either cell type. This pharmacological profile suggests that ATP release is not restricted to P-glycoprotein or the cystic fibrosis transmembrane conductance regulator, but can proceed through a route sensitive to NPPB. ATP release also was triggered by ionomycin through a different NPPB-insensitive mechanism, inhibitable by the calcium\/calmodulin-activated kinase II inhibitor KN-62. Thus, both layers of the ciliary epithelium store and release ATP, and purines likely modulate aqueous humor flow by paracrine and\/or autocrine mechanisms within the two cell layers of this epithelium.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.12.7174","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:44:19Z","timestamp":1027680259000},"page":"7174-7178","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":190,"title":["A release mechanism for stored ATP in ocular ciliary epithelial cells"],"prefix":"10.1073","volume":"95","author":[{"given":"Claire H.","family":"Mitchell","sequence":"first","affiliation":[{"name":"Departments of Physiology, Ophthalmology, and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Carr\u00e9","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Ophthalmology, and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice M.","family":"McGlinn","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Ophthalmology, and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Stone","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Ophthalmology, and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mortimer M.","family":"Civan","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Ophthalmology, and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,6,9]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"251","volume-title":"The Glaucomas","author":"Krupin T","year":"1995","unstructured":"T Krupin, M M Civan The Glaucomas, eds R Ritch, M B Shields, T Krupin (Mosby, 2nd Ed., St. Louis), pp. 251\u2013280 (1995).","edition":"2"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0017"},{"key":"e_1_3_3_3_2","first-page":"158","volume":"12","author":"Civan M M","year":"1997","unstructured":"M M Civan News Physiol Sci 12, 158\u2013162 (1997).","journal-title":"News Physiol Sci"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1993.1102"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.4.C1354"},{"key":"e_1_3_3_6_2","first-page":"320","volume":"273","author":"Crosson C E","year":"1995","unstructured":"C E Crosson J Pharmacol Exp Ther 273, 320\u2013326 (1995).","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_3_7_2","first-page":"1833","volume":"37","author":"Crosson C E","year":"1996","unstructured":"C E Crosson, T Gray Invest Ophthalmol Visual Sci 37, 1833\u20131839 (1996).","journal-title":"Invest Ophthalmol Visual Sci"},{"key":"e_1_3_3_8_2","unstructured":"Mitchell C. 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