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Leukocyte rolling velocities in cremaster muscle venules were increased significantly in ICAM-1-deficient mice during both trauma- and tumor necrosis factor \u03b1-induced inflammation, but rolling leukocyte flux was not reduced. Elimination of ICAM-1 expression in L-selectin-deficient mice resulted in a sharp reduction in the flux of rolling leukocytes during tumor necrosis factor \u03b1-induced inflammation. The observed differences in leukocyte rolling behavior demonstrated that ICAM-1 expression was required for optimal P- and L-selectin-mediated rolling. Increased leukocyte rolling velocities presumably translated into decreased tissue emigration because circulating neutrophil, monocyte, and lymphocyte numbers were increased markedly in L-selectin\/ICAM-1-deficient mice. Furthermore, neutrophil emigration during acute peritonitis was reduced by 80% in the double-deficient mice compared with either L-selectin or ICAM-1-deficient mice. Thus, members of the selectin and Ig families function synergistically to mediate optimal leukocyte rolling\n                    <jats:italic>in vivo<\/jats:italic>\n                    , which is essential for the generation of effective inflammatory responses.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.13.7562","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:42:40Z","timestamp":1027680160000},"page":"7562-7567","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":89,"title":["Optimal selectin-mediated rolling of leukocytes during inflammation\n                    <i>in vivo<\/i>\n                    requires intercellular adhesion molecule-1 expression"],"prefix":"10.1073","volume":"95","author":[{"given":"Douglas A.","family":"Steeber","sequence":"first","affiliation":[{"name":"Department of Immunology, Duke University Medical Center, Durham, NC 27710; and Department of 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