{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:37:16Z","timestamp":1781109436412,"version":"3.54.1"},"reference-count":54,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,7,7]]},"abstract":"<jats:p>\n                    The ciliary body contains an epithelial bilayer consisting of an outer pigmented cell layer (PE) and an inner nonpigmented cell layer (NPE) responsible for aqueous humor secretion. Secretion may be mediated in part by cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    ), but whether or how the two layers could coordinate their Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals to regulate secretion is unclear. To investigate interactions between PE and NPE, we examined [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    signaling in isolated intact ciliary epithelial bilayers using confocal microscopy. Phenylephrine selectively increased [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in PE and acetylcholine increased [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in NPE, but epinephrine increased [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in both layers. This increase spread from PE to NPE, and [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    signaling across the bilayer remained coordinated during [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    oscillations. All epinephrine-induced [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    signaling was blocked by the \u03b1\n                    <jats:sub>1<\/jats:sub>\n                    -adrenergic antagonist prazosin, whereas signaling in the NPE but not PE was blocked by the \u03b2-adrenergic antagonist propranolol, the gap junction blockers octanol and 18\u03b1-glycyrrhetinic acid, or the A kinase inhibitor\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>p<\/jats:sub>\n                    diastereomer of adenosine 3\u2032,5\u2032-cyclic monophosphothioate. The \u03b2-adrenergic agonist isoproterenol failed to increase Ca\n                    <jats:sup>2+<\/jats:sup>\n                    by itself, but isoproterenol plus phenylephrine-induced [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    signals across the bilayer similar to those induced by epinephrine. Finally, isoproterenol increased cell-to-cell spread of lucifer yellow via gap junctions, whereas cell-to-cell spread of [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    signals could be induced by photorelease of caged inositol 1,4,5-trisphosphate. Thus, calcium signals are coordinated in the epithelial bilayer so that adrenergic stimulation can increase [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in NPE, but only if NPE are primed by activation of endogenous adenylyl cyclase, whereupon they receive stimulation from adjacent PE via gap junctions. This novel interplay between endocrine and paracrine pathways may coordinate [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    signaling across the ciliary epithelial bilayer.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.14.8381","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:39:15Z","timestamp":1027679955000},"page":"8381-8386","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":26,"title":["Novel paracrine signaling mechanism in the ocular ciliary epithelium"],"prefix":"10.1073","volume":"95","author":[{"given":"Keiji","family":"Hirata","sequence":"first","affiliation":[{"name":"Departments of Ophthalmology, Medicine, and Cell Biology, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael 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