{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:36:32Z","timestamp":1791182192886,"version":"4.1.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,8,18]]},"abstract":"<jats:p>\n                    Hodgkin disease (HD) is characterized by a small number of malignant Hodgkin and Reed-Sternberg (H\/RS) cells among a major population of nonmalignant cells. The analysis of H\/RS cells has been hampered by their low frequency and fragility. Here, we describe the isolation of viable H\/RS cells from HD affected tissues by high gradient magnetic cell sorting (MACS) according to expression of CD30. The cells were enriched to a purity of up to 50%. H\/RS cells were distinguished from other CD30\n                    <jats:sup>+<\/jats:sup>\n                    cells by the expression of CD15, their size and granularity. No CD30\/CD15 double-positive cells could be enriched from a lymph node affected by the lymphocyte predominant subtype of HD, activated lymph nodes or peripheral blood of healthy donors. For two cases of HD individual MACS-purified H\/RS cells and H\/RS cells micromanipulated from tissue sections of the same lymphoma specimens were analyzed for Ig gene rearrangements. In both cases, identical V gene rearrangements were amplified from both sources of H\/RS cells, showing that H\/RS cells were successfully enriched. Moreover, the finding that in both cases no additional Ig gene rearrangements other than the ones identified in the H\/RS cells micromanipulated from tissue sections were amplified from the MACS-purified H\/RS cells further supports the monoclonality of these cells throughout the affected lymph nodes. The isolation of viable H\/RS cells ex vivo is prerequisite for a direct study of gene expression by those cells and of their interaction with cells in their vicinity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.17.10117","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"10117-10122","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":30,"title":["Isolation of viable Hodgkin and Reed-Sternberg cells from Hodgkin disease tissues"],"prefix":"10.1073","volume":"95","author":[{"given":"Johannes","family":"Irsch","sequence":"first","affiliation":[{"name":"Institute for Genetics, University of Cologne, 50931 Cologne, Germany; Miltenyi Biotec, 51429 Bergisch Gladbach, Germany; Institute for Pathology, University of Frankfurt, 60598 Frankfurt\/Main, Germany; Department of Internal Medicine I, University of Cologne, 50924 Cologne, Germany; and German Centre for Rheumatology Research, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Nitsch","sequence":"additional","affiliation":[{"name":"Institute for Genetics, University of Cologne, 50931 Cologne, Germany; Miltenyi Biotec, 51429 Bergisch Gladbach, Germany; Institute for Pathology, University of Frankfurt, 60598 Frankfurt\/Main, Germany; Department of Internal Medicine I, University of Cologne, 50924 Cologne, Germany; and German Centre for Rheumatology Research, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin-Leo","family":"Hansmann","sequence":"additional","affiliation":[{"name":"Institute for Genetics, University of Cologne, 50931 Cologne, Germany; Miltenyi Biotec, 51429 Bergisch Gladbach, Germany; Institute for Pathology, University of Frankfurt, 60598 Frankfurt\/Main, Germany; Department of Internal Medicine I, University of Cologne, 50924 Cologne, Germany; and German Centre for Rheumatology Research, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Rajewsky","sequence":"additional","affiliation":[{"name":"Institute for Genetics, University of Cologne, 50931 Cologne, Germany; Miltenyi Biotec, 51429 Bergisch Gladbach, Germany; Institute for Pathology, University of Frankfurt, 60598 Frankfurt\/Main, Germany; Department of Internal Medicine I, University of Cologne, 50924 Cologne, Germany; and German Centre for Rheumatology Research, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Tesch","sequence":"additional","affiliation":[{"name":"Institute for Genetics, University of Cologne, 50931 Cologne, Germany; Miltenyi Biotec, 51429 Bergisch Gladbach, Germany; Institute for Pathology, University of Frankfurt, 60598 Frankfurt\/Main, Germany; Department of Internal Medicine I, University of Cologne, 50924 Cologne, Germany; and German Centre for Rheumatology Research, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Diehl","sequence":"additional","affiliation":[{"name":"Institute for Genetics, University of Cologne, 50931 Cologne, Germany; Miltenyi Biotec, 51429 Bergisch Gladbach, Germany; Institute for Pathology, University of Frankfurt, 60598 Frankfurt\/Main, Germany; 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