{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:39:38Z","timestamp":1790192378516,"version":"4.1.0"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"18","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:p>Catecholamines, thought to derive from the extrinsic innervation of the ovary, participate in the regulation of ovarian development and mature gonadal function. Recently, intraovarian neurons containing tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, were described in the ovary of nonhuman primates. We now show that the primate ovary expresses both the genes encoding TH and dopamine \u03b2-hydroxylase (DBH), the key enzymes in norepinephrine (NE) biosynthesis. Ovarian neurons were identified as a site of TH and DBH gene expression, and surprisingly, oocytes were identified as an exclusive site of DBH synthesis. Oocytes contain neither TH mRNA nor protein, indicating that they are unable to synthesize dopamine (DA). They did, however, express a DA transporter gene identical to that found in human brain. The physiological relevance of this transporter system and DBH in oocytes was indicated by the ability of isolated oocytes to metabolize exogenous DA into NE. Isolated follicles containing oocytes\u2014but not those from which the oocytes had been removed\u2014responded to DA with an elevation in cAMP levels; this elevation was prevented by propranolol, a \u03b2-adrenoreceptor antagonist. The results suggest that oocytes and somatic cells are linked by a neuroendocrine loop consisting of NE synthesized in oocytes from actively transported DA and cAMP produced by somatic follicular cells in response to NE-induced \u03b2-adrenoreceptor activation.<\/jats:p>","DOI":"10.1073\/pnas.95.18.10990","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:52Z","timestamp":1027680052000},"page":"10990-10995","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":70,"title":["Oocytes are a source of catecholamines in the primate ovary: Evidence for a cell\u2013cell regulatory loop"],"prefix":"10.1073","volume":"95","author":[{"given":"Artur","family":"Mayerhofer","sequence":"first","affiliation":[{"name":"Divisions of Neuroscience and Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, OR 97006; Department of Molecular Anatomy, Anatomical Institute, Technical University, 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