{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:10:30Z","timestamp":1792030230391,"version":"4.4.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,9,15]]},"abstract":"<jats:p>\n                    The enzyme isopentenyl pyrophosphate (IPP) isomerase catalyzes the reversible isomerization of IPP to produce dimethylallyl pyrophosphate, the initial substrate leading to the biosynthesis of carotenoids and many other long-chain isoprenoids. Expression of IPP isomerase, and of two enzymes specific to the carotenoid pathway (lycopene \u03b2-cyclase and \u03b2-carotene-C-4-oxygenase), was followed in the green unicellular alga\n                    <jats:italic>Haematococcus pluvialis<\/jats:italic>\n                    after exposure to high illumination. This alga uniquely accumulates carotenoids in the cytoplasm and in late developmental stages turns deep-red in color because of accumulation of ketocarotenoids in the cytosol. The carotenoid\/chlorophyll ratio increased 3-fold in wild type and 6-fold in a precocious carotenoid-accumulating mutant (Car-3) within 24 h after increasing the illumination from 20 to 150 \u03bcmol photon m\n                    <jats:sup>\u22122<\/jats:sup>\n                    \u22c5s\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Two cDNAs encoding IPP isomerase in\n                    <jats:italic>Haematococcus<\/jats:italic>\n                    ,\n                    <jats:italic>ipiHp1<\/jats:italic>\n                    and\n                    <jats:italic>ipiHp2,<\/jats:italic>\n                    were identified. Although otherwise highly similar (95% identity overall), the predicted sequence of\n                    <jats:italic>ipiHp1<\/jats:italic>\n                    contained a 12-aa region not found in that of\n                    <jats:italic>ipiHp2<\/jats:italic>\n                    . This was reflected by a size difference between two polypeptides of 34 and 32.5 kDa, both of which reacted with an antibody to the product of\n                    <jats:italic>ipiHp1<\/jats:italic>\n                    . We suggest that the 32.5-kDa form is involved with the carotenoid accumulation in the cytoplasm, since the 32.5-kDa polypeptide was preferentially up-regulated by high light preceding the carotenoid increase and only this form was detected in red cysts.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.19.11482","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"11482-11488","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":127,"title":["Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte"],"prefix":"10.1073","volume":"95","author":[{"given":"Zairen","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, and Maryland Agricultural Experiment Station, University of Maryland, College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Francis X.","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, and Maryland Agricultural Experiment Station, University of Maryland, College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Gantt","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, and Maryland Agricultural Experiment Station, University of Maryland, College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,9,15]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"25","volume-title":"Target Sites of Herbicide Action","author":"Sandmann G","year":"1989","unstructured":"G Sandmann, P B\u00f6ger Target Sites of Herbicide Action, eds P B\u00f6ger, G Sandmann (CRC, Boca Raton, FL), pp. 25\u201344 (1989)."},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/978-94-011-2124-8_7","volume-title":"Carotenoids in Photosynthesis","author":"Demmig-Adams B","year":"1993","unstructured":"B Demmig-Adams, W W Adams Carotenoids in Photosynthesis, eds A J Young, G Britton (Chapman and Hall, London), pp. 206\u2013252 (1993)."},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0065-2296(08)60270-0","volume":"14","author":"Gray J C","year":"1987","unstructured":"J C Gray Adv Bot Res 14, 25\u201391 (1987).","journal-title":"Adv Bot Res"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1146\/annurev.pp.46.060195.002513","volume":"46","author":"Chappell J","year":"1995","unstructured":"J Chappell Annu Rev Plant Physiol Plant Mol Biol 46, 521\u2013547 (1995).","journal-title":"Annu Rev Plant Physiol Plant Mol Biol"},{"key":"e_1_3_4_5_2","first-page":"1015","volume":"7","author":"McGarvey D J","year":"1995","unstructured":"D J McGarvey, R Croteau Plant Cell 7, 1015\u20131026 (1995).","journal-title":"Plant Cell"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1146\/annurev.arplant.49.1.557","volume":"49","author":"Cunningham F X","year":"1998","unstructured":"F X Cunningham, E Gantt Annu Rev Plant Physiol Plant Mol Biol 49, 557\u2013583 (1998).","journal-title":"Annu Rev Plant Physiol Plant Mol Biol"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1432-1033.1994.tb18961.x","volume":"223","author":"Sandmann G","year":"1994","unstructured":"G Sandmann Eur J Biochem 223, 7\u201324 (1994).","journal-title":"Eur J Biochem"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1146\/annurev.micro.51.1.629","volume":"51","author":"Armstrong G A","year":"1997","unstructured":"G A Armstrong Annu Rev Microbiol 51, 629\u2013659 (1997).","journal-title":"Annu Rev Microbiol"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1104\/pp.105.2.529","volume":"105","author":"Albrecht M","year":"1994","unstructured":"M Albrecht, G Sandmann Plant Physiol 105, 529\u2013534 (1994).","journal-title":"Plant Physiol"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1039\/np9971400591","volume":"14","author":"Ramos-Valdivia A C","year":"1997","unstructured":"A C Ramos-Valdivia, R van der Heijden, R Verpoorte Nat Products Rep 14, 591\u2013603 (1997).","journal-title":"Nat Products Rep"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"24349","DOI":"10.1074\/jbc.271.40.24349","volume":"271","author":"Sun Z","year":"1996","unstructured":"Z Sun, E Gantt, F X Cunningham J Biol Chem 271, 24349\u201324352 (1996).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"18945","DOI":"10.1074\/jbc.272.30.18945","volume":"272","author":"Paton V G","year":"1997","unstructured":"V G Paton, J E Shackelford, S K Krisans J Biol Chem 272, 18945\u201318950 (1997).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"19169","DOI":"10.1016\/S0021-9258(19)47283-9","volume":"264","author":"Anderson M S","year":"1989","unstructured":"M S Anderson, M Muchlbacher, I P Street, J Proffitt, C D Poulter J Biol Chem 264, 19169\u201319175 (1989).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1006\/abbi.1996.0312","volume":"332","author":"Hahn F M","year":"1996","unstructured":"F M Hahn, J W Xuan, A F Chambers, C D Poulter Arch Biochem Biophys 332, 30\u201334 (1996).","journal-title":"Arch Biochem Biophys"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0003-9861(85)90042-6","volume":"240","author":"Bednarik D P","year":"1985","unstructured":"D P Bednarik, J K Hoober Arch Biochem Biophys 240, 369\u2013379 (1985).","journal-title":"Arch Biochem Biophys"},{"key":"e_1_3_4_16_2","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1111\/j.0022-3646.1995.00897.x","volume":"31","author":"Tan S","year":"1995","unstructured":"S Tan, F X Cunningham, M Youmans, B Grabowski, Z Sun, E Gantt J Phycol 31, 897\u2013905 (1995).","journal-title":"J Phycol"},{"key":"e_1_3_4_17_2","first-page":"1613","volume":"8","author":"Cunningham F X","year":"1996","unstructured":"F X Cunningham, B Pogson, Z Sun, K A McDonald, D DellaPenna, E Gantt Plant Cell 8, 1613\u20131626 (1996).","journal-title":"Plant Cell"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"9878","DOI":"10.1093\/nar\/16.20.9878","volume":"16","author":"Del Sal G","year":"1988","unstructured":"G Del Sal, G Manfioletti, C Schneider Nucleic Acids Res 16, 9878 (1988).","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli U K","year":"1970","unstructured":"U K Laemmli Nature (London) 227, 680\u2013685 (1970).","journal-title":"Nature (London)"},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/0003-2697(87)90409-X","volume":"162","author":"Peluso R W","year":"1987","unstructured":"R W Peluso, G H Rosenberg Anal Biochem 162, 389\u2013398 (1987).","journal-title":"Anal Biochem"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1104\/pp.94.1.334","volume":"94","author":"Mustardy L","year":"1990","unstructured":"L Mustardy, F X Cunningham, E Gantt Plant Physiol 94, 334\u2013340 (1990).","journal-title":"Plant Physiol"},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/BF00043657","volume":"29","author":"Kajiwara S","year":"1995","unstructured":"S Kajiwara, T Kakizono, T Saito, K Kondo, T Ohtani, N Nishio, S Nagai, N Misawa Plant Mol Biol 29, 343\u2013352 (1995).","journal-title":"Plant Mol Biol"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0015-3796(17)30778-3","volume":"167","author":"Jeffrey S W","year":"1975","unstructured":"S W Jeffrey, G F Humphey Biochem Physiol Pflanzen 167, 191\u2013194 (1975).","journal-title":"Biochem Physiol Pflanzen"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1111\/j.1399-3054.1965.tb06990.x","volume":"18","author":"Jaspers E M W","year":"1965","unstructured":"E M W Jaspers Physiol Plant 18, 933\u2013940 (1965).","journal-title":"Physiol Plant"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1529-8817.1968.tb04670.x","volume":"4","author":"Lang N","year":"1968","unstructured":"N Lang J Phycol 4, 12\u201319 (1968).","journal-title":"J Phycol"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1508\/cytologia.49.215","volume":"49","author":"Santos M F","year":"1984","unstructured":"M F Santos, J F Mesquita Cytologia 49, 215\u2013228 (1984).","journal-title":"Cytologia"},{"key":"e_1_3_4_27_2","first-page":"1107","volume":"6","author":"Cunningham F X","year":"1994","unstructured":"F X Cunningham, Z Sun, D Chamovitz, J Hirschberg, E Gantt Plant Cell 6, 1107\u20131121 (1994).","journal-title":"Plant Cell"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1104\/pp.108.2.855","volume":"108","author":"Blanc V M","year":"1995","unstructured":"V M Blanc, E Pichersky Plant Physiol 108, 855\u2013856 (1995).","journal-title":"Plant Physiol"},{"key":"e_1_3_4_29_2","first-page":"652","volume":"111","author":"Blanc V M","year":"1996","unstructured":"V M Blanc, K Mullin, E Pichersky Plant Physiol 111, 652 (1996).","journal-title":"Plant Physiol"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1023\/A:1005935516274","volume":"36","author":"Campbell M","year":"1998","unstructured":"M Campbell, F M Hahn, C D Poulter, T Leustek Plant Mol Biol 36, 323\u2013328 (1998).","journal-title":"Plant Mol Biol"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"4212","DOI":"10.1021\/bi00180a014","volume":"33","author":"Street I P","year":"1994","unstructured":"I P Street, H R Coffman, J A Baker, C D Poulter Biochemistry 33, 4212\u20134217 (1994).","journal-title":"Biochemistry"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1042\/bj3240421","volume":"324","author":"Kajiwara S","year":"1997","unstructured":"S Kajiwara, P D Fraser, K Kondo, N Misawa Biochem J 324, 421\u2013426 (1997).","journal-title":"Biochem J"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/0014-5793(95)00368-J","volume":"364","author":"Lotan T","year":"1995","unstructured":"T Lotan, J Hirschberg FEBS Lett 364, 125\u2013128 (1995).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1093\/oxfordjournals.pcp.a078171","volume":"32","author":"Boussiba S","year":"1991","unstructured":"S Boussiba, A Vonshak Plant Cell Physiol 32, 1077\u20131080 (1991).","journal-title":"Plant Cell Physiol"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2216\/i0031-8884-30-3-257.1","volume":"30","author":"Young Y Y R","year":"1991","unstructured":"Y Y R Young, Y K Lee Phycologia 30, 257\u2013261 (1991).","journal-title":"Phycologia"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.0022-3646.1994.00241.x","volume":"30","author":"Hagen C","year":"1994","unstructured":"C Hagen, W Braune, L O Bj\u00f6rn J Phycol 30, 241\u2013248 (1994).","journal-title":"J Phycol"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"18374","DOI":"10.1074\/jbc.270.31.18374","volume":"270","author":"Schroeder W A","year":"1995","unstructured":"W A Schroeder, E A Johnson J Biol Chem 270, 18374\u201318379 (1995).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/1011-1344(93)80145-Y","volume":"20","author":"Hagen C","year":"1993","unstructured":"C Hagen, W Braune, F Greulich J Photochem Photobiol B 20, 153\u2013160 (1993).","journal-title":"J Photochem Photobiol B"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/BF01279634","volume":"71","author":"Sprey B","year":"1970","unstructured":"B Sprey Protoplasma 71, 235\u2013250 (1970).","journal-title":"Protoplasma"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1751-1097.1996.tb03022.x","volume":"63","author":"Frank H A","year":"1996","unstructured":"H A Frank, R J Cogdell Photochem Photobiol 63, 257\u2013264 (1996).","journal-title":"Photochem Photobiol"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1128\/jb.178.3.619-624.1996","volume":"178","author":"Hahn H M","year":"1996","unstructured":"H M Hahn, J A Baker, C D Poulter J Bacteriol 178, 619\u2013624 (1996).","journal-title":"J Bacteriol"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.95.19.11482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T21:40:25Z","timestamp":1654551625000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.95.19.11482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9,15]]},"references-count":41,"journal-issue":{"issue":"19","published-print":{"date-parts":[[1998,9,15]]}},"alternative-id":["10.1073\/pnas.95.19.11482"],"URL":"https:\/\/doi.org\/10.1073\/pnas.95.19.11482","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9,15]]},"assertion":[{"value":"1998-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}