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U.S.A."],"published-print":{"date-parts":[[1998,10,27]]},"abstract":"<jats:p>\n                    To determine the role of intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in compaction, the first morphogenetic event in embryogenesis, we analyzed preimplantation mouse embryos under several decompacting conditions, including depletion of extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , blocking of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels, and inhibition of microfilaments, calmodulin, and intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release. Those treatments induced decompaction of mouse morulae and simultaneously induced changes in cytosolic free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration and deregionalization of E-cadherin and fodrin. When morulae were allowed to recompact, the location of both proteins recovered. In contrast, actin did not change its cortical location with compaction nor with decompaction-recompaction. Calmodulin localized in areas opposite to cell\u2013cell contacts in eight-cell stage embryos before and after compaction. Inhibition of calmodulin with trifluoperazine induced its delocalization while morulae decompacted. A nonspecific rise of intracellular free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    provoked by ionomycin did not affect the compacted shape. Moreover, the same decompacting treatments when applied to uncompacted embryos did not produce any change in intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Our results demonstrate that in preimplantation mouse embryos experimentally induced stage-specific changes of cell shape are accompanied by changes of intracellular free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and redistribution of the cytoskeleton-related proteins E-cadherin, fodrin, and calmodulin. We conclude that intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    specifically is involved in compaction and probably regulates the function and localization of cytoskeleton elements.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.22.12977","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"12977-12982","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":43,"title":["Increase of intracellular Ca\n                    <sup>2+<\/sup>\n                    and relocation of E-cadherin during experimental decompaction of mouse embryos"],"prefix":"10.1073","volume":"95","author":[{"given":"Roxana","family":"Pey","sequence":"first","affiliation":[{"name":"Departamento de Biologia and Centro de Equipamiento Mayor, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile; Department of Molecular Biology 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