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As a first step toward this goal, homologs of the eutherian amelogenin gene have been cloned and characterized in selected species of monotremes (platypus and echidna), reptiles (caiman), and amphibians (African clawed toad). Comparisons of the homologs reveal that the amelogenin gene evolves quickly in the repeat region, in which numerous insertions and deletions have obliterated any similarity among the genes, and slowly in other regions. The gene organization, the distribution of hydrophobic and hydrophilic segments in the encoded protein, and several other features have been conserved throughout the evolution of the tetrapod amelogenin gene. Clones corresponding to one locus only were found in caiman, whereas the clawed toad possesses at least two amelogenin-encoding loci.<\/jats:p>","DOI":"10.1073\/pnas.95.22.13056","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:36:44Z","timestamp":1027679804000},"page":"13056-13061","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":108,"title":["Identification and characterization of amelogenin genes in monotremes, reptiles, and amphibians"],"prefix":"10.1073","volume":"95","author":[{"given":"Satoru","family":"Toyosawa","sequence":"first","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biologie, Abteilung Immungenetik, Corrensstrasse 42, D-72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colm","family":"O\u2019hUigin","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biologie, Abteilung Immungenetik, Corrensstrasse 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