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The DNA also was detected but less frequently in the spleen, brain, and skin. There was a small but statistically significant increase in both collagen content and mineral content of bone 1 mo after the infusion. Similar results were obtained with infusion of relatively large amounts of wild-type whole marrow cells into the transgenic mice. In experiments in which male marrow stromal cells were infused into a female osteogenesis imperfecta-transgenic mouse, fluorescense\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization assays for the Y chromosome indicated that, after 2.5 mo, donor male cells accounted for 4\u201319% of the fibroblasts or fibroblast-like cells obtained in primary cultures of the lung, calvaria, cartilage, long bone, tail, and skin. In a parallel experiment in which whole marrow cells from a male mouse were infused into a female immunodeficient\n                    <jats:italic>rag<\/jats:italic>\n                    -\n                    <jats:italic>2<\/jats:italic>\n                    mouse, donor male cells accounted for 4\u20136% of the fibroblasts or fibroblast-like cells in primary cultures. The results support previous suggestions that marrow stromal cells or related cells in marrow serve as a source for continual renewal of cells in a number of nonhematopoietic tissues.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.3.1142","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:42:40Z","timestamp":1027680160000},"page":"1142-1147","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":448,"title":["Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a  phenotype of osteogenesis\u2009imperfecta"],"prefix":"10.1073","volume":"95","author":[{"given":"Ruth F.","family":"Pereira","sequence":"first","affiliation":[{"name":"Center for Gene Therapy, Departments of Radiation Oncology and Nuclear Medicine, and Pathology and Laboratory Medicine, Allegheny University of the Health Sciences, Philadelphia, PA 19102-1192; and Radiation Oncology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"O\u2019Hara","sequence":"additional","affiliation":[{"name":"Center for Gene Therapy, Departments of Radiation Oncology and Nuclear Medicine, and Pathology and Laboratory Medicine, Allegheny University of the Health Sciences, Philadelphia, PA 19102-1192; 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