{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:22:58Z","timestamp":1790832178840,"version":"4.1.0"},"reference-count":27,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,3,3]]},"abstract":"\n A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This \u201cdeoxyribozyme\u201d can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a\n k<\/jats:italic>\n obs<\/jats:sub>\n of 0.2 min\n \u22121<\/jats:sup>\n , whereas the corresponding uncatalyzed reaction could not be detected. The duplex and triplex recognition domains can be altered, making possible the targeted cleavage of single-stranded DNAs with different nucleotide sequences. Several small synthetic DNAs were made to function as simple \u201crestriction enzymes\u201d for the site-specific cleavage of single-stranded DNA.\n <\/jats:p>","DOI":"10.1073\/pnas.95.5.2233","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"2233-2237","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":238,"title":["Cleaving DNA with\u2009DNA"],"prefix":"10.1073","volume":"95","author":[{"given":"Nir","family":"Carmi","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shameelah R.","family":"Balkhi","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald R.","family":"Breaker","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,3,3]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.64.070195.003555"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr960009c"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(97)80105-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199511891"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0597-427"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-5521(94)90014-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-5521(95)90028-4"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.9.4262"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199628371"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1036"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(97)90244-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/375611a0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0996-743"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199713211"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(96)90170-2"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr960008k"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2468181"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi951907q"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(94)90008-6"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.64.070195.000433"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01056-6"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3118463"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2003222"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2195655"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(92)16029-J"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.24.9702"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.95.5.2233","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T16:25:56Z","timestamp":1649867156000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.95.5.2233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3,3]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,3,3]]}},"alternative-id":["10.1073\/pnas.95.5.2233"],"URL":"https:\/\/doi.org\/10.1073\/pnas.95.5.2233","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3,3]]},"assertion":[{"value":"1997-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-03-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}