{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:38:05Z","timestamp":1791077885086,"version":"4.1.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,3,31]]},"abstract":"<jats:p>\n                    Tumor necrosis factor-\u03b1 (TNF\u03b1) is a cytokine that induces protective inflammatory reactions and kills tumor cells but also causes severe damage when produced in excess, as in rheumatoid arthritis and septic shock. Soluble TNF\u03b1 is released from its membrane-bound precursor by a membrane-anchored proteinase, recently identified as a multidomain metalloproteinase called TNF\u03b1-converting enzyme or TACE. We have cocrystallized the catalytic domain of TACE with a hydroxamic acid inhibitor and have solved its 2.0 \u212b crystal structure. This structure reveals a polypeptide fold and a catalytic zinc environment resembling that of the snake venom metalloproteinases, identifying TACE as a member of the adamalysin\/ADAM family. However, a number of large insertion loops generate unique surface features. The pro-TNF\u03b1 cleavage site fits to the active site of TACE but seems also to be determined by its position relative to the base of the compact trimeric TNF\u03b1 cone. The active-site cleft of TACE shares properties with the matrix metalloproteinases but exhibits unique features such as a deep S3\u2032 pocket merging with the S1\u2032 specificity pocket below the surface. The structure thus opens a different approach toward the design of specific synthetic TACE inhibitors, which could act as effective therapeutic agents\n                    <jats:italic>in vivo<\/jats:italic>\n                    to modulate TNF\u03b1-induced pathophysiological effects, and might also help to control related shedding processes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.7.3408","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"3408-3412","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":334,"title":["Crystal structure of the catalytic domain of human tumor necrosis factor-\u03b1-converting enzyme"],"prefix":"10.1073","volume":"95","author":[{"given":"Klaus","family":"Maskos","sequence":"first","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Fernandez-Catalan","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Huber","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gleb P.","family":"Bourenkov","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Bartunik","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A.","family":"Ellestad","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranitha","family":"Reddy","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin F.","family":"Wolfson","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles T.","family":"Rauch","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly J.","family":"Castner","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Davis","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard R. G.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Petersen","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey N.","family":"Fitzner","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas Pat","family":"Cerretti","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl J.","family":"March","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond J.","family":"Paxton","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy A.","family":"Black","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram","family":"Bode","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Biochemie, D-82152 Martinsried, Germany; AG Proteindynamik MPG-ASMB c\/o DESY, D-22603 Hamburg, Germany; Wyeth\u2013Ayerst Research, Pearl River, NY 10965; and Immunex Corporation, Seattle, WA 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,3,31]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1615\/CritRevImmunol.v16.i1.10","volume":"16","author":"Bemelmans M H A","year":"1996","unstructured":"M H A Bemelmans, L J H VanTits, W A Buurman Crit Rev Immunol 16, 1\u201311 (1996).","journal-title":"Crit Rev Immunol"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/385729a0","volume":"385","author":"Black R A","year":"1997","unstructured":"R A Black, C T Rauch, C J Kozlosky, J J Peschon, J L Slack, M F Wolfson, B J Castner, K L Stocking, P Reddy, S Srinivasan, et al. Nature (London) 385, 729\u2013733 (1997).","journal-title":"Nature (London)"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/385733a0","volume":"385","author":"Moss M L","year":"1997","unstructured":"M L Moss, S L C Jin, M E Milla, W Burkhart, H L Carter, W J Chen, W C Clay, J R Didsberg, D Hassler, C R Hoffman, et al. Nature (London) 385, 733\u2013736 (1997).","journal-title":"Nature (London)"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/0014-5793(93)80312-I","volume":"331","author":"Bode W","year":"1993","unstructured":"W Bode, F-X Gomis-R\u00fcth, W St\u00f6cker FEBS Lett 331, 134\u2013140 (1993).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1002\/pro.5560040502","volume":"4","author":"St\u00f6cker W","year":"1995","unstructured":"W St\u00f6cker, F Grams, U Baumann, P Reinemer, F X Gomis-R\u00fcth, D B McKay, W Bode Protein Sci 4, 823\u2013840 (1995).","journal-title":"Protein Sci"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1002\/j.1460-2075.1993.tb06099.x","volume":"12","author":"Gomis-R\u00fcth F X","year":"1993","unstructured":"F X Gomis-R\u00fcth, L F Kress, W Bode EMBO J 12, 4151\u20134157 (1993).","journal-title":"EMBO J"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1006\/jmbi.1994.1392","volume":"239","author":"Gomis-R\u00fcth F X","year":"1994","unstructured":"F X Gomis-R\u00fcth, L F Kress, J Kellermann, I Mayr, X Lee, R Huber, W Bode J Mol Biol 239, 513\u2013544 (1994).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1006\/dbio.1996.0313","volume":"180","author":"Wolfsberg T G","year":"1996","unstructured":"T G Wolfsberg, J M White Dev Biol 180, 389\u2013401 (1996).","journal-title":"Dev Biol"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1006\/bbrc.1996.1186","volume":"225","author":"Black R A","year":"1996","unstructured":"R A Black, F H Durie, C Otten-Evans, R Miller, J L Slack, D H Lynch, B Castner, K M Mohler, M Gerhart, R S Johnson, et al. Biochem Biophys Res Commun 225, 400\u2013405 (1996).","journal-title":"Biochem Biophys Res Commun"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/370218a0","volume":"370","author":"Mohler K M","year":"1994","unstructured":"K M Mohler, P R Sleath, J N Fitzner, D P Cerretti, M Alderson, S S Kerwar, D S Torrance, C Otten-Evans, T Greenstreet, K Weerawarna, et al. Nature (London) 370, 218\u2013220 (1994).","journal-title":"Nature (London)"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s000110050175","volume":"46","author":"DiMartino M","year":"1997","unstructured":"M DiMartino, C Wolff, W High, G Stroup, S Hoffman, J Laydon, J C Lee, D Bertolini, W A Galloway, M J Crimmin, et al. Inflamm Res 46, 211\u2013215 (1997).","journal-title":"Inflamm Res"},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1084\/jem.181.3.1205","volume":"181","author":"Crowe P D","year":"1995","unstructured":"P D Crowe, B N Walter, K M Mohler, C Otten-Evans, R A Black, C F Ware J Exp Med 181, 1205\u20131210 (1995).","journal-title":"J Exp Med"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1056\/NEJM199707173370301","volume":"337","author":"Moreland L W","year":"1997","unstructured":"L W Moreland, S W Baumgartner, M H Schiff, E A Tindall, R M Fleischmann, A L Weaver, R E Ettlinger, S Cohen, W J Koopman, K Mohler, et al. N Engl J Med 337, 141\u2013147 (1997).","journal-title":"N Engl J Med"},{"key":"e_1_3_4_14_2","volume-title":"DENZO: A Film Processing for Macromolecular Crystallography","author":"Otwinowski Z","year":"1993","unstructured":"Z Otwinowski, W Minor DENZO: A Film Processing for Macromolecular Crystallography (Yale University, New Haven, CT, 1993)."},{"key":"e_1_3_4_15_2","volume-title":"Isomorphous Replacement and Anomalous Scattering, Daresbury Study Weekend Proceedings","author":"Otwinowski Z","year":"1991","unstructured":"Z Otwinowski Isomorphous Replacement and Anomalous Scattering, Daresbury Study Weekend Proceedings (SERC Daresbury Laboratory, Warrington, U.K., 1991)."},{"key":"e_1_3_4_16_2","unstructured":"Cowtan K. (1994) Joint CCP 4 ESF-EACBM Newsletter on Protein Crystallography 31 ."},{"key":"e_1_3_4_17_2","volume-title":"turbo-frodo in Silicon Graphics Geometry, Partners Directory","author":"Roussel A","year":"1989","unstructured":"A Roussel, C Cambilleau turbo-frodo in Silicon Graphics Geometry, Partners Directory (Silicon Graphics, Mountain View, CA, 1989)."},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/0959-440X(91)90100-8","volume":"1","author":"Br\u00fcnger A T","year":"1991","unstructured":"A T Br\u00fcnger Curr Opin Struct Biol 1, 1016\u20131022 (1991).","journal-title":"Curr Opin Struct Biol"},{"key":"e_1_3_4_19_2","first-page":"760","volume":"50","year":"1994","unstructured":"Acta Crystallogr D50, 760\u2013763 (1994).","journal-title":"Acta Crystallogr"},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/0263-7855(93)87009-T","volume":"11","author":"Evans S V","year":"1993","unstructured":"S V Evans J Mol Graph 11, 134\u2013138 (1993).","journal-title":"J Mol Graph"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/37995","volume":"389","author":"Gomis-R\u00fcth F X","year":"1997","unstructured":"F X Gomis-R\u00fcth, K Maskos, M Betz, A Bergner, R Huber, K Suzuki, N Yoshida, H Nagase, K Brew, G P Bourenkov, et al. Nature (London) 389, 77\u201381 (1997).","journal-title":"Nature (London)"},{"key":"e_1_3_4_22_2","first-page":"A166","volume":"64","author":"Nicholls A","year":"1993","unstructured":"A Nicholls, R Bharadwaj, B Honig Biophys J 64, A166 (1993).","journal-title":"Biophys J"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"24558","DOI":"10.1074\/jbc.272.39.24588","volume":"272","author":"Rosendahl M S","year":"1997","unstructured":"M S Rosendahl, S C Ko, D L Long, M T Brewer, B Rosenzweig, E Hedl, L Anderson, S M Pyle, J Moreland, M A Meyers, et al. J Biol Chem 272, 24558\u201324593 (1997).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1111\/j.1432-1033.1995.tb20329.x","volume":"228","author":"Grams F","year":"1995","unstructured":"F Grams, P Reinemer, J C Powers, T Kleine, M Pieper, H Tschesche, R Huber, W Bode Eur J Biochem 228, 830\u2013841 (1995).","journal-title":"Eur J Biochem"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"8216","DOI":"10.1021\/bi952182t","volume":"35","author":"Tang P","year":"1996","unstructured":"P Tang, M-C Hung, J Klostergaard Biochemistry 35, 8216\u20138225 (1996).","journal-title":"Biochemistry"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"8226","DOI":"10.1021\/bi952183l","volume":"35","author":"Tang P","year":"1996","unstructured":"P Tang, M-C Hung, J Klostergaard Biochemistry 35, 8226\u20138233 (1996).","journal-title":"Biochemistry"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0092-8674(90)90158-B","volume":"63","author":"Perez C","year":"1990","unstructured":"C Perez, I Albert, K DeFay, N Zachariades, L Gooding, M Kriegler Cell 63, 251\u2013258 (1990).","journal-title":"Cell"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/338225a0","volume":"338","author":"Jones E Y","year":"1989","unstructured":"E Y Jones, D I Stuart, N P C B Walker Nature (London) 338, 225\u2013228 (1989).","journal-title":"Nature (London)"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"8447","DOI":"10.1073\/pnas.91.18.8447","volume":"91","author":"Zhang D","year":"1994","unstructured":"D Zhang, I Botos, F X Gomis-R\u00fcth, R Doll, C Blood, F G Njoroge, J W Fox, W Bode, E F Meyer Proc Natl Acad Sci USA 91, 8447\u20138451 (1994).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1093\/oxfordjournals.jbchem.a021215","volume":"119","author":"Kumasaka T","year":"1996","unstructured":"T Kumasaka, M Yamamoto, H Moriyama, N Tanaka, M Sato, Y Katsube, Y Yamakawa, T Omori-Satoh, S Iwanaga, T Ueki J Biochem 119, 49\u201357 (1996).","journal-title":"J Biochem"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0014-5793(96)01410-X","volume":"400","author":"Lunn C A","year":"1997","unstructured":"C A Lunn, X Fan, B Dalie, K Miller, P J Zavodny, S W Narula, D Lundell FEBS Lett 400, 333\u2013335 (1997).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0092-8674(00)80335-9","volume":"90","author":"Pan D","year":"1997","unstructured":"D Pan, G M Rubin Cell 90, 271\u2013280 (1997).","journal-title":"Cell"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1042\/bj3210265","volume":"321","author":"Hooper N M","year":"1997","unstructured":"N M Hooper, E H Karran, A J Turner Biochem J 321, 265\u2013279 (1997).","journal-title":"Biochem J"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.95.7.3408","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T22:56:54Z","timestamp":1654556214000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.95.7.3408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3,31]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1998,3,31]]}},"alternative-id":["10.1073\/pnas.95.7.3408"],"URL":"https:\/\/doi.org\/10.1073\/pnas.95.7.3408","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3,31]]},"assertion":[{"value":"1998-01-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-03-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}