{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:45:44Z","timestamp":1791935144167,"version":"4.3.4"},"reference-count":22,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,3,31]]},"abstract":"<jats:p>\n                    The mosquito\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    is the world\u2019s most important vector of yellow fever and dengue viruses. Work is currently in progress to control the transmission of these viruses by genetically altering the capacity of wild\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    populations to support virus replication. The germ-line transformation system reported here constitutes a major advance toward the implementation of this control strategy. A modified\n                    <jats:italic>Hermes<\/jats:italic>\n                    transposon carrying a 4.7-kb fragment of genomic DNA that includes a wild-type allele of the\n                    <jats:italic>Drosophila melanogaster cinnabar (cn)<\/jats:italic>\n                    gene was used to transform a white-eyed recipient strain of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    . Microinjection of preblastoderm mosquito embryos with this construct resulted in 50% of the emergent G\n                    <jats:sub>0<\/jats:sub>\n                    adults showing some color in their eyes. Three transformed families were recovered, each resulting from an independent insertion event of the\n                    <jats:italic>cn<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    -carrying transposon. The\n                    <jats:italic>cn<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    gene functioned as a semidominant transgene and segregated in Mendelian ratios.\n                    <jats:italic>Hermes<\/jats:italic>\n                    shows great promise as a vector for efficient, heritable, and stable transformation of this important mosquito vector species.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.7.3743","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:44Z","timestamp":1027679504000},"page":"3743-3747","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":290,"title":["Stable transformation of the yellow fever mosquito,\n                    <i>Aedes aegypti<\/i>\n                    , with the\n                    <i>Hermes<\/i>\n                    element from the housefly"],"prefix":"10.1073","volume":"95","author":[{"given":"Nijole","family":"Jasinskiene","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig J.","family":"Coates","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark Q.","family":"Benedict","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Cornel","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Salazar","family":"Rafferty","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A.","family":"James","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank H.","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341-3724"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,3,31]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"52","volume":"3","author":"Collins F H","year":"1996","unstructured":"F H Collins, A A James Sci Med 3, 52\u201361 (1996).","journal-title":"Sci Med"},{"key":"e_1_3_3_2_2","first-page":"S1:113","volume":"65","author":"Meredith S E O","year":"1990","unstructured":"S E O Meredith, A A James Ann Parasitol Humaine Comp 65, S1:113\u2013118 (1990).","journal-title":"Ann Parasitol Humaine Comp"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3039658"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.502"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1989.tb00467.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.456"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00756-3"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5263.884"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(97)00018-0"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a111606"},{"key":"e_1_3_3_11_2","first-page":"381","volume":"28","author":"Bhalla S C","year":"1968","unstructured":"S C Bhalla Mosq News 28, 381\u2013385 (1968).","journal-title":"Mosq News"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(97)00084-2"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00057589"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300032699"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/142.3.907"},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/978-94-009-1535-0_35","volume-title":"The Molecular Biology of Insect Vectors of Disease","author":"Morris A","year":"1997","unstructured":"A Morris The Molecular Biology of Insect Vectors of Disease, eds J M Crampton, C B Beard, C Louis (Chapman & Hall, London), pp. 423\u2013429 (1997)."},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/978-94-009-1535-0_2","volume-title":"The Molecular Biology of Insect Vectors of Disease","author":"Munstermann L E","year":"1997","unstructured":"L E Munstermann The Molecular Biology of Insect Vectors of Disease, eds J M Crampton, C B Beard, C Louis (Chapman & Hall, London), pp. 13\u201320 (1997)."},{"key":"e_1_3_3_18_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989","unstructured":"J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. 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