{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:38:18Z","timestamp":1792651098286,"version":"4.5.0"},"reference-count":16,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,3,31]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic>mariner<\/jats:italic>\n                    transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito,\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    . To determine if this demonstrated mobility could be utilized to genetically transform the mosquito, a modified\n                    <jats:italic>mariner<\/jats:italic>\n                    element marked with a wild-type allele of the\n                    <jats:italic>Drosophila melanogaster cinnabar<\/jats:italic>\n                    gene was microinjected into embryos of a kynurenine hydroxylase-deficient, white-eyed recipient strain. Three of 69 fertile male founders resulting from the microinjected embryos produced families with colored-eyed progeny individuals, a transformation rate of 4%. The transgene-mediated complementation of eye color was observed to segregate in a Mendelian manner, although one insertion segregates with the recessive allele (female-determining) of the sex-determining locus, and a separate insertion is homozygous lethal. Molecular analysis of selected transformed families demonstrated that a single complete copy of the construct had integrated independently in each case and that it had done so in a transposase-mediated manner. The availability of a\n                    <jats:italic>mariner<\/jats:italic>\n                    transformation system greatly enhances our ability to study and manipulate this important vector species.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.7.3748","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:31:44Z","timestamp":1027679504000},"page":"3748-3751","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":305,"title":["<i>Mariner<\/i>\n                    transposition and transformation of the yellow fever mosquito,\n                    <i>Aedes aegypti<\/i>"],"prefix":"10.1073","volume":"95","author":[{"given":"Craig J.","family":"Coates","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nijole","family":"Jasinskiene","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Miyashiro","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A.","family":"James","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,3,31]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"52","volume":"3","author":"Collins F H","year":"1996","unstructured":"F H Collins, A A James Sci Med 3, 52\u201361 (1996).","journal-title":"Sci Med"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","unstructured":"Jasinskiene N. 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