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U.S.A."],"published-print":{"date-parts":[[1998,3,31]]},"abstract":"<jats:p>\n                    In this study, we demonstrate that: (\n                    <jats:italic>i<\/jats:italic>\n                    ) injection of an adenovirus (Ad) vector containing the brain-derived neurotrophic factor (BDNF) gene (Ad.BDNF) into the vitreous chamber of adult rats results in selective transgene expression by M\u00fcller cells; (\n                    <jats:italic>ii<\/jats:italic>\n                    )\n                    <jats:italic>in vitro<\/jats:italic>\n                    , M\u00fcller cells infected with Ad.BDNF secrete BDNF that enhances neuronal survival; (\n                    <jats:italic>iii<\/jats:italic>\n                    )\n                    <jats:italic>in vivo<\/jats:italic>\n                    , Ad-mediated expression of functional BDNF by M\u00fcller cells, temporarily extends the survival of axotomized retinal ganglion cells (RGCs); 16 days after axotomy, injured retinas treated with Ad.BDNF showed a 4.5-fold increase in surviving RGCs compared with control retinas; (\n                    <jats:italic>iv<\/jats:italic>\n                    ) the transient expression of the BDNF transgene, which lasted \u224810 days, can be prolonged with immunosuppression for at least 30 days, and such Ad-mediated BDNF remains biologically active, (\n                    <jats:italic>v<\/jats:italic>\n                    ) persistent expression of BDNF by infected M\u00fcller cells does not further enhance the survival of injured RGCs, indicating that the effect of this neurotrophin on RGC survival is limited by changes induced by the lesion within 10\u201316 days after optic nerve transection rather than the availability of BDNF. Thus, Ad-transduced M\u00fcller cells are a novel pathway for sustained delivery of BDNF to acutely-injured RGCs. Because these cells span the entire thickness of the retina, Ad-mediated gene delivery to M\u00fcller cells may also be useful to influence photoreceptors and other retinal neurons.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.7.3978","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"3978-3983","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":318,"title":["Prolonged delivery of brain-derived neurotrophic factor by adenovirus-infected M\u00fcller cells temporarily rescues injured retinal ganglion cells"],"prefix":"10.1073","volume":"95","author":[{"given":"Adriana","family":"Di Polo","sequence":"first","affiliation":[{"name":"Centre for Research in Neuroscience, The Montreal General Hospital Research Institute and McGill University, Montr\u00e9al, PQ, Canada H3G 1A4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwig 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