{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:55:03Z","timestamp":1791147303469,"version":"4.1.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1999,5,11]]},"abstract":"<jats:p>\n                    Mice lacking the chemokine stromal cell-derived factor\/pre-B cell growth stimulating factor or its primary physiological receptor CXCR4 revealed defects in B lymphopoiesis and bone marrow myelopoiesis during embryogenesis. We show here that adoptive transfer experiments reveal a deficiency in long-term lymphoid and myeloid repopulation in adult bone marrow by CXCR4\u2212\/\u2212 fetal liver cells, although stromal cell-derived factor\/pre-B cell growth stimulating factor\u2212\/\u2212 fetal liver cells yield normal multilineage reconstitution. These findings indicate that CXCR4 is required cell autonomously for lymphoid and myeloid repopulation in bone marrow. In addition, CXCR4\u2212\/\u2212 fetal liver cells generated much more severely reduced numbers of B cells relative to other lineages in bone marrow. Furthermore, the repopulation of c-kit\n                    <jats:sup>+<\/jats:sup>\n                    Sca-1\n                    <jats:sup>+<\/jats:sup>\n                    lin\n                    <jats:sup>low\/\u2212<\/jats:sup>\n                    cells by CXCR4\u2212\/\u2212 fetal liver cells was less affected compared with c-kit\n                    <jats:sup>+<\/jats:sup>\n                    Sca-1\n                    <jats:sup>\u2212<\/jats:sup>\n                    lin\n                    <jats:sup>low\/\u2212<\/jats:sup>\n                    cells. By previous studies, it has been shown that c-kit\n                    <jats:sup>+<\/jats:sup>\n                    Sca-1\n                    <jats:sup>+<\/jats:sup>\n                    lin\n                    <jats:sup>low\/\u2212<\/jats:sup>\n                    cells are highly purified primitive hematopoietic progenitors and that c-kit\n                    <jats:sup>+<\/jats:sup>\n                    Sca-1\n                    <jats:sup>\u2212<\/jats:sup>\n                    lin\n                    <jats:sup>low\/\u2212<\/jats:sup>\n                    cells are more committed hematopoietic progenitors in mice. Thus, CXCR4 may play an essential role in generation and\/or expansion of early hematopoietic progenitors within bone marrow.\n                  <\/jats:p>","DOI":"10.1073\/pnas.96.10.5663","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"5663-5667","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":188,"title":["A cell-autonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution"],"prefix":"10.1073","volume":"96","author":[{"given":"Kenji","family":"Kawabata","sequence":"first","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Ujikawa","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Egawa","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kawamoto","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunobu","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisashi","family":"Iizasa","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimoto","family":"Katsura","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadamitsu","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Nagasawa","sequence":"additional","affiliation":[{"name":"Department of Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan; Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Pharmaceutics, Kyoritsu College of Pharmacy, 1-5-30 Shibakouen, Minato-ku, Tokyo 105-8512, Japan; and Osaka University, 2-1 Yamada-oka, Suita-city, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1999,5,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60403-4"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.675"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8342023"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.6.2305"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5263.872"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/382829a0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/382833a0"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14726"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/31261"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/31269"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.16.9448"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/382635a0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.1.111"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270729"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.1.100.100_100_110"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.3.756"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.3.539"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.8.1257"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.5.1213"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.12.3044.3044"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.12.4781"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.2.518"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90167-1"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.3.1195"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5403.845"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.19.9125"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.1.257"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.5.1955"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.4.1519"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.23.7019"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.8.1383"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.8.1389"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.8.1395"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0198-072"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.96.10.5663","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:57:38Z","timestamp":1649865458000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.96.10.5663"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5,11]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1999,5,11]]}},"alternative-id":["10.1073\/pnas.96.10.5663"],"URL":"https:\/\/doi.org\/10.1073\/pnas.96.10.5663","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,5,11]]},"assertion":[{"value":"1999-03-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-05-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}